Detailed information NTG0336017

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EQH50_RS14520
• Genome accession: NZ_CP035207
• Coordinates: 2968552..2969352 (+)
• Length: 266 a.a.
• NCBI ID: WP_023319753.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae strain TH114
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2963552..2974352

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH50_RS14485 (EQH50_14655)	gspH	2963705..2964220 (+)	516	WP_004204416.1	type II secretion system minor pseudopilin GspH	-
EQH50_RS14490 (EQH50_14660)	gspI	2964217..2964582 (+)	366	WP_004204415.1	type II secretion system minor pseudopilin GspI	-
EQH50_RS14495 (EQH50_14665)	gspJ	2964579..2965175 (+)	597	WP_032426022.1	type II secretion system minor pseudopilin GspJ	-
EQH50_RS14500 (EQH50_14670)	gspK	2965172..2966152 (+)	981	WP_130943003.1	type II secretion system minor pseudopilin GspK	-
EQH50_RS14505 (EQH50_14675)	gspL	2966145..2967341 (+)	1197	WP_152651530.1	type II secretion system protein GspL	-
EQH50_RS14510 (EQH50_14680)	-	2967334..2967819 (+)	486	WP_004204409.1	type II secretion system protein M	-
EQH50_RS14515 (EQH50_14685)	-	2967809..2968537 (+)	729	WP_152651531.1	type II secretion system protein N	-
EQH50_RS14520 (EQH50_14690)	pilD	2968552..2969352 (+)	801	WP_023319753.1	A24 family peptidase	Machinery gene
EQH50_RS14525 (EQH50_14695)	hrpB	2969400..2971829 (-)	2430	WP_152651532.1	ATP-dependent helicase HrpB	-
EQH50_RS14530 (EQH50_14700)	thpR	2971902..2972438 (+)	537	WP_004204405.1	RNA 2',3'-cyclic phosphodiesterase	-
EQH50_RS14535 (EQH50_14705)	sfsA	2972438..2973154 (+)	717	WP_004204404.1	DNA/RNA nuclease SfsA	-
EQH50_RS14540 (EQH50_14710)	dksA	2973317..2973772 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29352.89 Da
• Isoelectric Point: 8.8894

>NTDB_id=336017 EQH50_RS14520 WP_023319753.1 2968552..2969352(+) (pilD) [Klebsiella quasipneumoniae strain TH114]
MTTLAALSLHFPLIWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLFASASGLTWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=336017 EQH50_RS14520 WP_023319753.1 2968552..2969352(+) (pilD) [Klebsiella quasipneumoniae strain TH114]
ATGACGACATTAGCCGCATTATCCCTGCATTTCCCTCTTATCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCTTGTCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCACCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCTGGTTATCTGGCGCTGTGGTCGGTTTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCACAGGTGCTGTTGTTCGCCTCGGCCAGCGGCCTGACGTGGACCCTGCTGCAGCGCCTGTGGACCAGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-230)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425