Detailed information NTG0336002

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EQH49_RS10845
• Genome accession: NZ_CP035202
• Coordinates: 2217754..2218554 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain LH94
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2212754..2223554

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH49_RS10825 (EQH49_11000)	dksA	2213334..2213789 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
EQH49_RS10830 (EQH49_11005)	sfsA	2213952..2214668 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
EQH49_RS10835 (EQH49_11010)	thpR	2214668..2215204 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
EQH49_RS10840 (EQH49_11015)	hrpB	2215277..2217706 (+)	2430	WP_152650686.1	ATP-dependent helicase HrpB	-
EQH49_RS10845 (EQH49_11020)	pilD	2217754..2218554 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
EQH49_RS10850 (EQH49_11025)	-	2218551..2219297 (-)	747	WP_004145897.1	type II secretion system protein N	-
EQH49_RS10855 (EQH49_11030)	-	2219287..2219772 (-)	486	WP_023312675.1	type II secretion system protein M	-
EQH49_RS10860 (EQH49_11035)	gspL	2219765..2220961 (-)	1197	WP_064145608.1	type II secretion system protein GspL	-
EQH49_RS10865 (EQH49_11040)	gspK	2220948..2221934 (-)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
EQH49_RS10870 (EQH49_11045)	gspJ	2221931..2222527 (-)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
EQH49_RS10875 (EQH49_11050)	gspI	2222524..2222889 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
EQH49_RS10880 (EQH49_11055)	gspH	2222886..2223401 (-)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=336002 EQH49_RS10845 WP_004145898.1 2217754..2218554(-) (pilD) [Klebsiella pneumoniae strain LH94]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=336002 EQH49_RS10845 WP_004145898.1 2217754..2218554(-) (pilD) [Klebsiella pneumoniae strain LH94]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTATGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425