Detailed information NTG0335975

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EP126_RS16460
• Genome accession: NZ_CP035196
• Coordinates: 3350658..3351458 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain LH375
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3345658..3356458

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EP126_RS16425 (EP126_16580)	gspH	3345814..3346329 (+)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-
EP126_RS16430 (EP126_16585)	gspI	3346326..3346691 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
EP126_RS16435 (EP126_16590)	gspJ	3346688..3347284 (+)	597	WP_032422017.1	type II secretion system minor pseudopilin GspJ	-
EP126_RS16440 (EP126_16595)	gspK	3347281..3348267 (+)	987	WP_023282192.1	type II secretion system minor pseudopilin GspK	-
EP126_RS16445 (EP126_16600)	gspL	3348254..3349447 (+)	1194	WP_004213440.1	type II secretion system protein GspL	-
EP126_RS16450 (EP126_16605)	-	3349440..3349925 (+)	486	WP_004151943.1	type II secretion system protein M	-
EP126_RS16455 (EP126_16610)	-	3349915..3350661 (+)	747	WP_004145897.1	type II secretion system protein N	-
EP126_RS16460 (EP126_16615)	pilD	3350658..3351458 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
EP126_RS16465 (EP126_16620)	hrpB	3351506..3353935 (-)	2430	WP_032422018.1	ATP-dependent helicase HrpB	-
EP126_RS16470 (EP126_16625)	thpR	3354008..3354544 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
EP126_RS16475 (EP126_16630)	sfsA	3354544..3355260 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
EP126_RS16480 (EP126_16635)	dksA	3355423..3355878 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=335975 EP126_RS16460 WP_004145898.1 3350658..3351458(+) (pilD) [Klebsiella pneumoniae strain LH375]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=335975 EP126_RS16460 WP_004145898.1 3350658..3351458(+) (pilD) [Klebsiella pneumoniae strain LH375]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425