Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYA05_RS19945 Genome accession   NZ_CP043211
Coordinates   4065472..4065912 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli O16:H48 strain PG20180050     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4060472..4070912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA05_RS19925 (FYA05_19925) aroP 4061470..4062843 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FYA05_RS19930 (FYA05_19930) ampE 4062886..4063740 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FYA05_RS19935 (FYA05_19935) ampD 4063737..4064288 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYA05_RS19940 (FYA05_19940) nadC 4064376..4065269 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYA05_RS19945 (FYA05_19945) pilA 4065472..4065912 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FYA05_RS19950 (FYA05_19950) pilB 4065922..4067307 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  FYA05_RS19955 (FYA05_19955) hofC 4067297..4068499 (+) 1203 WP_000157266.1 protein transport protein HofC -
  FYA05_RS19960 (FYA05_19960) guaC 4068534..4069577 (-) 1044 WP_001217338.1 GMP reductase -
  FYA05_RS23030 - 4069733..4069777 (-) 45 WP_120795372.1 protein YacM -
  FYA05_RS19965 (FYA05_19965) coaE 4069802..4070422 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=335667 FYA05_RS19945 WP_000360904.1 4065472..4065912(+) (pilA) [Escherichia coli O16:H48 strain PG20180050]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=335667 FYA05_RS19945 WP_000360904.1 4065472..4065912(+) (pilA) [Escherichia coli O16:H48 strain PG20180050]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment