Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQI48_RS00580 Genome accession   NZ_CP035164
Coordinates   105613..106989 (+) Length   458 a.a.
NCBI ID   WP_029727024.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM104005     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100613..111989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI48_RS00560 (EQI48_00560) ctsR 100966..101430 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  EQI48_RS00565 (EQI48_00565) mcsA 101444..102001 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  EQI48_RS00570 (EQI48_00570) mcsB 102001..103092 (+) 1092 WP_003235007.1 protein arginine kinase -
  EQI48_RS00575 (EQI48_00575) clpC 103089..105521 (+) 2433 WP_003235011.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EQI48_RS00580 (EQI48_00580) radA 105613..106989 (+) 1377 WP_029727024.1 DNA repair protein RadA Machinery gene
  EQI48_RS00585 (EQI48_00585) disA 106993..108075 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  EQI48_RS00590 (EQI48_00590) yacL 108191..109291 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  EQI48_RS00595 (EQI48_00595) ispD 109306..110004 (+) 699 WP_046664350.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EQI48_RS00600 (EQI48_00600) ispF 109997..110473 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49452.96 Da        Isoelectric Point: 8.2399

>NTDB_id=335162 EQI48_RS00580 WP_029727024.1 105613..106989(+) (radA) [Bacillus subtilis strain SRCM104005]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANIAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=335162 EQI48_RS00580 WP_029727024.1 105613..106989(+) (radA) [Bacillus subtilis strain SRCM104005]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTAACGAAAGAAGGGTCTATTGCAGGTCCAAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTCTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATATTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627

Multiple sequence alignment