Detailed information NTG0335073

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: EQH95_RS20485
• Genome accession: NZ_CP035162
• Coordinates: 3816222..3817367 (+)
• Length: 381 a.a.
• NCBI ID: WP_069837832.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103886
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3811222..3822367

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH95_RS20460 (EQH95_20460)	albF	3811242..3812522 (+)	1281	WP_069837829.1	pitrilysin family protein	-
EQH95_RS20465 (EQH95_20465)	albG	3812519..3813220 (+)	702	WP_069837830.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
EQH95_RS20470 (EQH95_20470)	ywhL	3813226..3814602 (-)	1377	WP_069837831.1	YncE family protein	-
EQH95_RS20475 (EQH95_20475)	ywhK	3814641..3815992 (-)	1352	Protein_3953	YncE family protein	-
EQH95_RS23265 (EQH95_20480)	-	3815989..3816219 (-)	231	Protein_3954	hypothetical protein	-
EQH95_RS20485 (EQH95_20485)	rapF	3816222..3817367 (+)	1146	WP_069837832.1	response regulator aspartate phosphatase RapF	Regulator
EQH95_RS20490 (EQH95_20490)	phrF	3817351..3817470 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
EQH95_RS20495 (EQH95_20495)	-	3817568..3817995 (+)	428	Protein_3957	YbaK/EbsC family protein	-
EQH95_RS20500 (EQH95_20500)	speB	3818060..3818932 (-)	873	WP_003227545.1	agmatinase	-
EQH95_RS20505 (EQH95_20505)	speE	3818993..3819823 (-)	831	WP_003227543.1	spermidine synthase	-
EQH95_RS20510 (EQH95_20510)	pbpG	3820025..3822100 (+)	2076	WP_069837833.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45624.07 Da
• Isoelectric Point: 5.0624

>NTDB_id=335073 EQH95_RS20485 WP_069837832.1 3816222..3817367(+) (rapF) [Bacillus subtilis strain SRCM103886]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFTIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=335073 EQH95_RS20485 WP_069837832.1 3816222..3817367(+) (rapF) [Bacillus subtilis strain SRCM103886]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACAGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACATATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCACTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.688	100	0.987
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57