Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   EPZ47_RS25415 Genome accession   NZ_CP035088
Coordinates   5724107..5725696 (+) Length   529 a.a.
NCBI ID   WP_135847228.1    Uniprot ID   -
Organism   Pseudomonas viciae strain 11K1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5719107..5730696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPZ47_RS25395 (EPZ47_25390) pgeF 5720906..5721631 (-) 726 WP_135847225.1 peptidoglycan editing factor PgeF -
  EPZ47_RS25400 (EPZ47_25395) rluD 5721628..5722590 (-) 963 WP_003205696.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EPZ47_RS25405 (EPZ47_25400) - 5722742..5723758 (+) 1017 WP_135847226.1 outer membrane protein assembly factor BamD -
  EPZ47_RS25410 (EPZ47_25405) - 5723884..5724117 (+) 234 WP_135847227.1 PP0621 family protein -
  EPZ47_RS25415 (EPZ47_25410) pilS 5724107..5725696 (+) 1590 WP_135847228.1 two-component system sensor histidine kinase NtrB Regulator
  EPZ47_RS25420 (EPZ47_25415) pilR 5725693..5727039 (+) 1347 WP_135847229.1 sigma-54-dependent transcriptional regulator Regulator
  EPZ47_RS25425 (EPZ47_25420) - 5727125..5727490 (-) 366 WP_135847230.1 DUF6124 family protein -
  EPZ47_RS25430 (EPZ47_25425) thiO 5728272..5729372 (-) 1101 WP_135847231.1 glycine oxidase ThiO -
  EPZ47_RS25435 (EPZ47_25430) - 5729427..5729828 (-) 402 WP_135847232.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58800.46 Da        Isoelectric Point: 7.0115

>NTDB_id=334341 EPZ47_RS25415 WP_135847228.1 5724107..5725696(+) (pilS) [Pseudomonas viciae strain 11K1]
MIAEASSPRVKQAQRLLRLYHLYRLSIGITLVLLISSKLDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGAPSAIGNLLIVSVAIGNTLLRGRIGLLIAAVATIGIVGSSFFLDLGDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRQRRVQLANESALNLLGMHDLVGQQLD
DYSIALVESLQLWRNNPSLRPPSLTVTSTGLTLQPSFIALGHNEQHQILVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNDADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRAWLEQFVRQAQENASEH
QQLHVSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAQVHEQAEAWLKLFIDPHSQLSTLDIIDNGPGVTPDQQVHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=334341 EPZ47_RS25415 WP_135847228.1 5724107..5725696(+) (pilS) [Pseudomonas viciae strain 11K1]
GTGATCGCTGAGGCCTCGAGCCCCCGCGTCAAACAGGCGCAGCGCCTGCTGCGCCTCTATCACCTGTATCGCCTGAGCAT
CGGCATCACCCTGGTGCTGTTGATCTCCAGCAAACTGGACAATCGTCTGCTGGAGTTCGCCAACGACGACCTGCTGCGCA
GCGGTAGCTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTCCTTGAGAACACTCGCCGCCCTGCCCGGCTGTTT
GGCCTGGCACTCACCGACGTGCTGTTGCTCTCGTGGCTGTTCTTCGCCGCCGGCGGCGCTCCCAGCGCCATCGGCAACCT
GCTCATTGTTTCGGTGGCCATCGGCAATACGCTACTGCGAGGCCGCATCGGCCTGTTGATCGCCGCTGTCGCCACGATCG
GTATCGTCGGCTCGAGTTTCTTCCTGGACCTCGGCGATTCGAACCGTCCCAGCAGTTATCTGCAGGCCGGAACCCTGGGG
GCGCTGTGCTTTGCCGCGGCCCTGTTGGTACAAGGCCTGACCCGGCGACTGGAAGCCAGCGAAACCCTGGCCGAGCAACG
CGCCAGCGAAGTGATCGGCCTCGAAGCGCTCAATGCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTCGACC
GCCAGCGTCGGGTGCAATTGGCCAACGAGAGCGCGCTGAACCTGCTGGGCATGCACGATCTGGTCGGCCAGCAGCTCGAC
GATTACTCCATCGCCCTGGTCGAAAGCCTGCAACTGTGGCGCAACAACCCCAGCCTGCGCCCGCCCAGCCTGACCGTTAC
CAGCACGGGCCTGACCCTGCAACCGAGCTTCATCGCCTTGGGCCACAACGAGCAACATCAGATCCTGGTCTTTCTCGAAG
ACCTGGCCCAGGTTGCCCAGCAGGCCCAGCAACTGAAACTCGCCTCCCTCGGGCGCCTCACCGCCGGCATCGCCCACGAA
ATCCGCAACCCCTTGGGCGCCATCAGCCACGCGGCGCAATTACTGCGCGAATCCGAGGAACTGAACGACGCGGATCGACG
TCTGACGCAGATTATTCAAGATCACTCCCAACGAATGAATCGCGTCATTGAAAACGTCCTGCAGCTGTCTCGCCGCCAGC
AAACCACGCCGCAGCGCCTGGACTTGCGGGCCTGGCTCGAGCAATTCGTGCGTCAGGCCCAAGAAAACGCAAGCGAGCAC
CAGCAGTTGCACGTGAGCATCGACCCAGGCGACTACACCACGCTGATGGACCCCGACCAGTTGACGCAAGTGCTCGACAA
CCTGCTACGCAACGCCTGGCGCCACAGCGCGCAGGTCCATGAACAGGCCGAGGCCTGGCTGAAACTGTTCATCGATCCCC
ATAGCCAGTTATCCACGCTGGACATCATCGACAACGGCCCTGGCGTGACGCCGGACCAGCAGGTGCATTTGTTCGAACCC
TTCTTCACCACCAGCAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACTTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.618

97.921

0.594

Multiple sequence alignment