Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EMO94_RS03625 Genome accession   NZ_CP035005
Coordinates   664696..665193 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain PCFH-226     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 659696..670193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EMO94_RS03585 - 659741..660727 (+) 987 WP_000161315.1 ROK family glucokinase -
  EMO94_RS03590 - 660727..661056 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EMO94_RS03595 - 661053..661676 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EMO94_RS03600 comGA 661728..662702 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EMO94_RS03605 comGB 662674..663744 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  EMO94_RS03610 comGC 663766..664068 (+) 303 WP_029657948.1 competence type IV pilus major pilin ComGC Machinery gene
  EMO94_RS03615 comGD 664046..664492 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  EMO94_RS03620 comGE 664479..664778 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  EMO94_RS03625 comGF 664696..665193 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  EMO94_RS03630 - 665290..665436 (+) 147 WP_001789879.1 hypothetical protein -
  EMO94_RS03635 - 665426..665950 (+) 525 WP_001015120.1 shikimate kinase -
  EMO94_RS03640 gcvT 666109..667200 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EMO94_RS03645 gcvPA 667220..668566 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EMO94_RS03650 gcvPB 668559..670031 (+) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=333932 EMO94_RS03625 WP_001825340.1 664696..665193(+) (comGF) [Staphylococcus aureus strain PCFH-226]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=333932 EMO94_RS03625 WP_001825340.1 664696..665193(+) (comGF) [Staphylococcus aureus strain PCFH-226]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment