Detailed information NTG0333617

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: EO946_RS18900
• Genome accession: NZ_CP034943
• Coordinates: 3672299..3673444 (+)
• Length: 381 a.a.
• NCBI ID: WP_003222022.1
• Uniprot ID: -
• Organism: Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3667299..3678444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EO946_RS18875 (EO946_18870)	-	3667434..3668594 (+)	1161	WP_079996665.1	insulinase family protein	-
EO946_RS18880 (EO946_18875)	-	3668599..3669882 (+)	1284	WP_003222013.1	pitrilysin family protein	-
EO946_RS18885 (EO946_18880)	albG	3669879..3670580 (+)	702	WP_003222015.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
EO946_RS18890 (EO946_18885)	-	3670717..3672072 (-)	1356	WP_003222017.1	YncE family protein	-
EO946_RS18900 (EO946_18895)	rapF	3672299..3673444 (+)	1146	WP_003222022.1	response regulator aspartate phosphatase RapF	Regulator
EO946_RS18905 (EO946_18900)	phrF	3673428..3673547 (+)	120	WP_003222023.1	phosphatase RapF inhibitor PhrF	Regulator
EO946_RS18910 (EO946_18905)	speB	3673700..3674572 (-)	873	WP_003222025.1	agmatinase	-
EO946_RS18915 (EO946_18910)	speE	3674633..3675463 (-)	831	WP_003222027.1	spermidine synthase	-
EO946_RS18920 (EO946_18915)	-	3675665..3677740 (+)	2076	WP_003222029.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45659.13 Da
• Isoelectric Point: 5.5270

>NTDB_id=333617 EO946_RS18900 WP_003222022.1 3672299..3673444(+) (rapF) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MTGVISSSSVGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGVLDYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFKKAYSMAKAENQPQLMGRTLYNIGLCKNSQSQYED
AVTYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHAYHSKGMAYSQKAGDSIYLSEFEFLKSLYLSGPDEEAI
QRFFDFLESKMLYADLEDFALDVAKYYHERENFQKASAYFLKVEQVRQLIQGGVRLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=333617 EO946_RS18900 WP_003222022.1 3672299..3673444(+) (rapF) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
GTGACAGGTGTCATATCTTCTTCTTCCGTCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGAAGAGAAATCAAACAAGAGCTGGATCAAATGGAAGAAGACCAAGATCTTCATTTGTACTATT
CACTGATGGAATTCCGGCACAACCTGATGCTTGAGTACCTTGAACCACTAGAGAAAATGCGGATCGAGGAACAGCCGAGA
TTGTCAGATTTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGAGTCCTTGATTATTACTTTAATTTCTTCAG
AGGCATGTATGAGCTAGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTTAAAAAGGCCGAAAGCAAGTTGATATTTG
TTAAGGATCGAATAGAGAAGGCAGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAGGCATATGAAATATACAAAGAACATGAAGCTTACAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCTACCAATTTCTTAGATTTAAAACAATATGAGGATGCCATCTCACATTTTAAAAAAGCTTACTCTATGGCGAAAG
CTGAGAATCAGCCCCAATTAATGGGGAGAACATTGTACAACATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCGTAACATATTTCAAAAGAGCAATAGCTGTTTTTGAGGAATCTAACATTCTTCCTTCCCTGCCCCAAGCCTATTTTTT
AATTACACAAATTCATTATAAATTGGGAAAAATGGATAAAGCCCATGCATATCATAGTAAGGGAATGGCTTATTCTCAAA
AGGCCGGAGATTCAATCTATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCTGATGAAGAAGCAATT
CAAAGGTTCTTTGATTTCCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTCTTGATGTGGCAAAATATTA
TCATGAACGTGAAAATTTTCAAAAAGCTTCTGCTTATTTCCTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGATTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-207)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	96.063	100	0.961
rapC	Bacillus subtilis subsp. subtilis str. 168	57.632	99.738	0.575