Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EO946_RS02015 Genome accession   NZ_CP034943
Coordinates   369800..370249 (-) Length   149 a.a.
NCBI ID   WP_136975843.1    Uniprot ID   -
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364800..375249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS20930 - 365208..365335 (+) 128 Protein_340 hypothetical protein -
  EO946_RS01980 (EO946_01980) - 365332..365787 (+) 456 WP_003224877.1 RDD family protein -
  EO946_RS01985 (EO946_01985) - 365870..366202 (+) 333 WP_003224878.1 YckD family protein -
  EO946_RS01990 (EO946_01990) - 366337..366759 (+) 423 WP_003224879.1 MarR family winged helix-turn-helix transcriptional regulator -
  EO946_RS01995 (EO946_01995) - 366747..367088 (+) 342 WP_003224880.1 DUF3147 family protein -
  EO946_RS02000 (EO946_02000) - 367108..367836 (+) 729 WP_003224881.1 AAA family ATPase -
  EO946_RS02005 (EO946_02005) bglC 367909..369342 (+) 1434 WP_003224917.1 6-phospho-beta-glucosidase -
  EO946_RS02010 (EO946_02010) nin/comJ 369379..369777 (-) 399 WP_003224919.1 DNA-entry nuclease inhibitor Regulator
  EO946_RS02015 (EO946_02015) nucA/comI 369800..370249 (-) 450 WP_136975843.1 DNA-entry nuclease Machinery gene
  EO946_RS02020 (EO946_02020) tlpC 370423..372147 (-) 1725 WP_003224925.1 methyl-accepting chemotaxis protein TlpC -
  EO946_RS02025 (EO946_02025) hxlB 372260..372817 (-) 558 WP_003224927.1 6-phospho-3-hexuloisomerase -
  EO946_RS02030 (EO946_02030) hxlA 372822..373454 (-) 633 WP_003224929.1 3-hexulose-6-phosphate synthase -
  EO946_RS02035 (EO946_02035) hxlR 373685..374047 (+) 363 WP_003224931.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16392.32 Da        Isoelectric Point: 4.3043

>NTDB_id=333551 EO946_RS02015 WP_136975843.1 369800..370249(-) (nucA/comI) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MNITMDIIKTILLVIVVIAAAAVGLIKGDFFSADQKTSQTEEYDETIALPSDRYPETAKHIKDAINEGHSDVCTIDRDGA
EERREQSLKDVPPKTGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=333551 EO946_RS02015 WP_136975843.1 369800..370249(-) (nucA/comI) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
GTGAACATCACGATGGACATCATAAAAACGATACTTCTCGTCATCGTCGTCATAGCAGCTGCAGCTGTCGGCCTGATCAA
GGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACTGAAGAATATGACGAAACAATTGCCCTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAACATATTAAGGATGCGATAAATGAGGGGCACTCTGATGTGTGCACCATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAAGACGTACCGCCAAAAACGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCTGCTGACAACCGCGGTGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGACTATCCAGACGGCACAAAGGTTTTATTCACGATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

93.96

100

0.94

Multiple sequence alignment