Detailed information NTG0333529

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EO763_RS15175
• Genome accession: NZ_CP034938
• Coordinates: 3373604..3374449 (-)
• Length: 281 a.a.
• NCBI ID: WP_161529454.1
• Uniprot ID: -
• Organism: Pectobacterium odoriferum strain JK2.1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3368604..3379449

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EO763_RS15150 (EO763_15380)	-	3368765..3369535 (-)	771	WP_161529450.1	ABC transporter ATP-binding protein	-
EO763_RS15155 (EO763_15385)	-	3369537..3370574 (-)	1038	WP_161529451.1	iron ABC transporter permease	-
EO763_RS15160 (EO763_15390)	-	3370571..3371635 (-)	1065	WP_161529452.1	ABC transporter substrate-binding protein	-
EO763_RS15165 (EO763_15395)	-	3371987..3373012 (+)	1026	WP_161529453.1	LLM class flavin-dependent oxidoreductase	-
EO763_RS15170 (EO763_15400)	ypdK	3373369..3373443 (+)	75	WP_099606229.1	membrane protein YpdK	-
EO763_RS15175 (EO763_15405)	pilD	3373604..3374449 (-)	846	WP_161529454.1	A24 family peptidase	Machinery gene
EO763_RS15180 (EO763_15410)	-	3374539..3375285 (-)	747	WP_161529455.1	type II secretion system protein N	-
EO763_RS15185 (EO763_15415)	-	3375275..3375769 (-)	495	WP_161529456.1	type II secretion system protein M	-
EO763_RS15190 (EO763_15420)	gspL	3375766..3377043 (-)	1278	WP_161529457.1	type II secretion system protein GspL	-
EO763_RS15195 (EO763_15425)	gspK	3377040..3378026 (-)	987	WP_161529458.1	type II secretion system minor pseudopilin GspK	-
EO763_RS15200 (EO763_15430)	gspJ	3378023..3378673 (-)	651	WP_161529459.1	type II secretion system minor pseudopilin GspJ	-
EO763_RS15205 (EO763_15435)	gspI	3378673..3379038 (-)	366	WP_044208462.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31329.22 Da
• Isoelectric Point: 6.4529

>NTDB_id=333529 EO763_RS15175 WP_161529454.1 3373604..3374449(-) (pilD) [Pectobacterium odoriferum strain JK2.1]
MDDLREFAQLFPAWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLVFLAAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTTKPLAFGPWLAIGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=333529 EO763_RS15175 WP_161529454.1 3373604..3374449(-) (pilD) [Pectobacterium odoriferum strain JK2.1]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGGGCACTGGGCGTGTTGGGGTTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAGCAGGCTATT
GCGGTAAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGATCCCGCTGCTGCCATCAATCGGTGTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGTTTTTCTGGCGGCAGGTTTGCTCTGGTTACCCGGTATGGCGCTGT
GGGGGGCGTTGATCCTGCTGTCATTCCTGCTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTTTCACTGAACGATGCCGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCAACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGAGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTTTGGTGGCAGCGTTGAGC
GGTCTGGTCGTGACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGACTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATCGGCGGGGTGTTCGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.292	96.441	0.495
pilD	Vibrio campbellii strain DS40M4	46.667	96.085	0.448
pilD	Neisseria gonorrhoeae MS11	46.36	92.883	0.431
pilD	Acinetobacter baumannii D1279779	43.223	97.153	0.42
pilD	Acinetobacter nosocomialis M2	42.857	97.153	0.416