Detailed information NTG0332975

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ENZ43_RS23315
• Genome accession: NZ_CP034778
• Coordinates: 4326919..4327719 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 18CPO060
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4321919..4332719

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ENZ43_RS23280 (ENZ43_23280)	gspH	4322075..4322590 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
ENZ43_RS23285 (ENZ43_23285)	gspI	4322587..4322952 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
ENZ43_RS23290 (ENZ43_23290)	gspJ	4322949..4323545 (+)	597	WP_022615423.1	type II secretion system minor pseudopilin GspJ	-
ENZ43_RS23295 (ENZ43_23295)	gspK	4323542..4324528 (+)	987	WP_004213439.1	type II secretion system minor pseudopilin GspK	-
ENZ43_RS23300 (ENZ43_23300)	gspL	4324515..4325708 (+)	1194	WP_004213440.1	type II secretion system protein GspL	-
ENZ43_RS23305 (ENZ43_23305)	-	4325701..4326186 (+)	486	WP_004151943.1	type II secretion system protein M	-
ENZ43_RS23310 (ENZ43_23310)	-	4326176..4326922 (+)	747	WP_004145897.1	type II secretion system protein N	-
ENZ43_RS23315 (ENZ43_23315)	pilD	4326919..4327719 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
ENZ43_RS23320 (ENZ43_23320)	hrpB	4327767..4330196 (-)	2430	WP_004213442.1	ATP-dependent helicase HrpB	-
ENZ43_RS23325 (ENZ43_23325)	thpR	4330269..4330805 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
ENZ43_RS23330 (ENZ43_23330)	sfsA	4330805..4331521 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ENZ43_RS23335 (ENZ43_23335)	dksA	4331684..4332139 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=332975 ENZ43_RS23315 WP_004213441.1 4326919..4327719(+) (pilD) [Klebsiella pneumoniae strain 18CPO060]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=332975 ENZ43_RS23315 WP_004213441.1 4326919..4327719(+) (pilD) [Klebsiella pneumoniae strain 18CPO060]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425