Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EKQ63_RS02240 Genome accession   NZ_CP034686
Coordinates   68711..70087 (+) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus sp. BD59S     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 63711..75087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKQ63_RS02220 (EKQ63_02220) ctsR 63905..64366 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  EKQ63_RS02225 (EKQ63_02225) - 64540..65088 (+) 549 WP_087951326.1 UvrB/UvrC motif-containing protein -
  EKQ63_RS02230 (EKQ63_02230) - 65093..66166 (+) 1074 WP_087951327.1 protein arginine kinase -
  EKQ63_RS02235 (EKQ63_02235) clpC 66179..68614 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EKQ63_RS02240 (EKQ63_02240) radA 68711..70087 (+) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  EKQ63_RS02245 (EKQ63_02245) disA 70091..71164 (+) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  EKQ63_RS02250 (EKQ63_02250) - 71325..72434 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  EKQ63_RS02255 (EKQ63_02255) ispD 72451..73131 (+) 681 WP_143880702.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EKQ63_RS02260 (EKQ63_02260) ispF 73247..73723 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=332473 EKQ63_RS02240 WP_001085212.1 68711..70087(+) (radA) [Bacillus sp. BD59S]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=332473 EKQ63_RS02240 WP_001085212.1 68711..70087(+) (radA) [Bacillus sp. BD59S]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGGCGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTGATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTGTTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTCCTATATATATCAGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCGATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGCCTTGCAGAAGTATTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCAGTTGGGGTTGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCGCGAAGAATGGCAACGGGAATTGATCATAATCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCAACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAACTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment