Detailed information NTG0033226

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SPP_RS04745
• Genome accession: NC_012467
• Coordinates: 880236..882476 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942420.1
• Uniprot ID: A0A4J1ZPJ8
• Organism: Streptococcus pneumoniae P1031
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 875236..887476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPP_RS04710 (SPP_0955)	-	875399..876367 (+)	969	WP_000658180.1	PhoH family protein	-
SPP_RS04715 (SPP_0956)	-	876547..877047 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
SPP_RS04720 (SPP_0957)	-	877050..877376 (+)	327	Protein_901	TfoX/Sxy family protein	-
SPP_RS11905 (SPP_0958)	ald	877677..878788 (-)	1112	Protein_902	alanine dehydrogenase	-
SPP_RS04735 (SPP_0960)	-	878965..879534 (+)	570	WP_000443745.1	GNAT family N-acetyltransferase	-
SPP_RS04740 (SPP_0961)	comEA/celA/cilE	879602..880252 (+)	651	WP_000387352.1	helix-hairpin-helix domain-containing protein	Machinery gene
SPP_RS04745 (SPP_0962)	comEC/celB	880236..882476 (+)	2241	WP_000942420.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SPP_RS04750	-	882655..882843 (+)	189	WP_001812421.1	hypothetical protein	-
SPP_RS04755 (SPP_0963)	-	882876..883463 (+)	588	WP_000939895.1	ATP-binding cassette domain-containing protein	-
SPP_RS04760 (SPP_0964)	-	883467..884651 (+)	1185	WP_000655953.1	hypothetical protein	-
SPP_RS04765 (SPP_0965)	infC	884964..885494 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
SPP_RS04770 (SPP_0966)	rpmI	885527..885727 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
SPP_RS04775 (SPP_0967)	rplT	885779..886138 (+)	360	WP_000124836.1	50S ribosomal protein L20	-
SPP_RS04780 (SPP_0968)	-	886196..886576 (+)	381	WP_000157154.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84639.13 Da
• Isoelectric Point: 9.4311

>NTDB_id=33226 SPP_RS04745 WP_000942420.1 880236..882476(+) (comEC/celB) [Streptococcus pneumoniae P1031]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCLFIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQN
LVDSVERVRILPDTIKVNGDSLSFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
LKTQGIYQTLNIKRIQSFQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLVFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLISLALVYDLRKNIKGLTVLSLLITGLFFLTK
YPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
ENVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQQGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGVDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=33226 SPP_RS04745 WP_000942420.1 880236..882476(+) (comEC/celB) [Streptococcus pneumoniae P1031]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTGTTACTATGGCTTTACTACGCCATTTT
CTCAGTATCCTATTTTGCTTTGTTGGGTTTTGTTTTTCTGCTAGTCTGCCTCTTTATCCAATTTCCTTGGAAATCAGCAG
GTAAAGTTCTAGTGATTTGTGGAGTCTTTGGCTTCTGGTTTCTGTTTCAAACTTGGCAACAGAGTCAAGTGAGTCAAAAT
TTGGTGGATTCTGTTGAAAGGGTACGGATTTTACCAGACACTATTAAGGTTAACGGTGACAGTCTGTCCTTTCGTGGTAA
GTCTGATGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACTGACC
TTCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTGACTACCAAGGCTAT
CTGAAGACTCAGGGAATTTACCAGACACTCAATATTAAAAGAATCCAGTCATTCCAAAAGGTTGGCAGTTGGGATATAGG
TGAAAACCTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGATGCACTTCCCAGACCCTATGCGCAATTACATGA
CAGGACTCTTGCTAGGACATCTGGACACCGATTTTGAGGAGATGAATGAGCTTTATTCCAGTTTAGGAATTATTCACCTT
TTTGCCTTGTCAGGTATGCAGGTAGGGTTTTTCATGGACGGATTTAAGAAACTACTTTTACGATTGGGCTTGACACAAGA
AAAGTTGAAGTGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGTGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTTTTTGACTTGGTCTTCTTACCGCTCTTGTCTATC
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTAGAGGGCATTATTCGCTTGGT
CTCGCAGGTGGCAAGGAGACCGCTTGTCTTTGGTCAACCCAACGCATGGCTTTTAATCTTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGGAAAAACATTAAAGGATTAACAGTATTGAGTTTATTGATTACAGGTCTCTTTTTCCTTACCAAG
TATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGAATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACAAGGAAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGGTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (30-176)

• Competence protein (Competence) (216-458)

3D structure

• AlphaFold DB: A0A4J1ZPJ8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	96.917	100	0.969
comEC/celB	Streptococcus pneumoniae Rx1	95.174	100	0.952
comEC/celB	Streptococcus pneumoniae D39	95.174	100	0.952
comEC/celB	Streptococcus pneumoniae R6	95.174	100	0.952
comEC/celB	Streptococcus mitis SK321	92.091	100	0.921
comEC/celB	Streptococcus mitis NCTC 12261	91.812	99.866	0.917
comEC	Lactococcus lactis subsp. cremoris KW2	44.534	99.33	0.442