Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EJ379_RS21705 Genome accession   NZ_CP034551
Coordinates   4187797..4188840 (-) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus cereus ATCC 14579     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4182797..4193840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ379_RS21660 (EJ379_21655) - 4183230..4183430 (-) 201 WP_000106083.1 YqzE family protein -
  EJ379_RS21665 (EJ379_21660) - 4183469..4183966 (-) 498 WP_000836663.1 shikimate kinase -
  EJ379_RS21670 (EJ379_21665) - 4184082..4184732 (-) 651 WP_000183861.1 2OG-Fe(II) oxygenase -
  EJ379_RS21675 (EJ379_21670) comGG 4184907..4185278 (-) 372 WP_001231316.1 competence type IV pilus minor pilin ComGG -
  EJ379_RS21680 (EJ379_21675) comGF 4185275..4185745 (-) 471 WP_033685282.1 competence type IV pilus minor pilin ComGF -
  EJ379_RS21685 (EJ379_21680) - 4185715..4186017 (-) 303 WP_000829469.1 hypothetical protein -
  EJ379_RS21690 (EJ379_21685) comGD 4186010..4186465 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  EJ379_RS21695 (EJ379_21690) comGC 4186462..4186761 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  EJ379_RS21700 (EJ379_21695) comGB 4186773..4187810 (-) 1038 WP_011110378.1 competence type IV pilus assembly protein ComGB -
  EJ379_RS21705 (EJ379_21700) comGA 4187797..4188840 (-) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  EJ379_RS21710 (EJ379_21705) - 4189046..4189741 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  EJ379_RS21715 (EJ379_21710) - 4189867..4190109 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  EJ379_RS21720 (EJ379_21715) - 4190217..4191611 (+) 1395 WP_001094344.1 L-cystine transporter -
  EJ379_RS21725 (EJ379_21720) - 4191690..4192082 (-) 393 WP_000875399.1 hypothetical protein -
  EJ379_RS21730 (EJ379_21725) - 4192170..4192385 (-) 216 WP_001008327.1 DUF3912 family protein -
  EJ379_RS21735 (EJ379_21730) - 4192638..4192949 (+) 312 WP_001093237.1 hypothetical protein -
  EJ379_RS21740 (EJ379_21735) - 4192984..4193472 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=331872 EJ379_RS21705 WP_001013170.1 4187797..4188840(-) (comGA) [Bacillus cereus ATCC 14579]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=331872 EJ379_RS21705 WP_001013170.1 4187797..4188840(-) (comGA) [Bacillus cereus ATCC 14579]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372

Multiple sequence alignment