Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EJN89_RS13475 Genome accession   NZ_CP034441
Coordinates   2569116..2569802 (+) Length   228 a.a.
NCBI ID   WP_001793467.1    Uniprot ID   A0A641AAY3
Organism   Staphylococcus aureus strain PMB 81-4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2564116..2574802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN89_RS13440 yqeH 2564643..2565743 (+) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -
  EJN89_RS13445 aroE 2565757..2566563 (+) 807 WP_000666748.1 shikimate dehydrogenase -
  EJN89_RS13450 yhbY 2566567..2566857 (+) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  EJN89_RS13455 nadD 2566860..2567429 (+) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  EJN89_RS13460 yqeK 2567419..2568003 (+) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EJN89_RS13465 rsfS 2568004..2568357 (+) 354 WP_001088022.1 ribosome silencing factor -
  EJN89_RS13470 - 2568360..2569076 (+) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  EJN89_RS13475 comEA 2569116..2569802 (+) 687 WP_001793467.1 ComEA family DNA-binding protein Machinery gene
  EJN89_RS13480 - 2569894..2570355 (+) 462 WP_000439693.1 ComE operon protein 2 -
  EJN89_RS13485 comEC 2570360..2572561 (+) 2202 WP_001566670.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EJN89_RS13490 holA 2572618..2573592 (+) 975 WP_001282568.1 DNA polymerase III subunit delta -
  EJN89_RS13495 rpsT 2573637..2573888 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25786.50 Da        Isoelectric Point: 9.6753

>NTDB_id=331316 EJN89_RS13475 WP_001793467.1 2569116..2569802(+) (comEA) [Staphylococcus aureus strain PMB 81-4]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=331316 EJN89_RS13475 WP_001793467.1 2569116..2569802(+) (comEA) [Staphylococcus aureus strain PMB 81-4]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATAATG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A641AAY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

100

100

1
  comEA Staphylococcus aureus N315

97.368

100

0.974

Multiple sequence alignment