Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SPJ_RS00215 Genome accession   NC_012466
Coordinates   32201..33562 (+) Length   453 a.a.
NCBI ID   WP_075213698.1    Uniprot ID   A0A7X2XL61
Organism   Streptococcus pneumoniae JJA     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 12371..44471 32201..33562 within 0


Gene organization within MGE regions


Location: 12371..44471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPJ_RS00065 (SPJ_0013) ftsH 12371..14329 (+) 1959 WP_000744545.1 ATP-dependent zinc metalloprotease FtsH -
  SPJ_RS00070 (SPJ_0014) comX/comX2 14451..14930 (+) 480 WP_000588897.1 sigma-70 family RNA polymerase sigma factor Regulator
  SPJ_RS11325 - 20422..21268 (+) 847 Protein_14 IS630 family transposase -
  SPJ_RS11330 - 21303..22100 (-) 798 Protein_15 transposase -
  SPJ_RS00135 (SPJ_0027) comW 22366..22602 (+) 237 WP_000939544.1 sigma(X)-activator ComW Regulator
  SPJ_RS00140 (SPJ_0028) - 22833..24119 (+) 1287 WP_000205044.1 adenylosuccinate synthase -
  SPJ_RS00145 (SPJ_0029) tadA 24320..24787 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  SPJ_RS13150 (SPJ_0030) - 24996..25694 (-) 699 WP_001106362.1 site-specific integrase -
  SPJ_RS13155 (SPJ_0031) - 25784..26131 (-) 348 WP_001839379.1 hypothetical protein -
  SPJ_RS00160 (SPJ_0032) - 26192..27262 (-) 1071 WP_000401841.1 type I restriction endonuclease -
  SPJ_RS00165 (SPJ_0033) - 27279..27659 (-) 381 WP_000170931.1 ImmA/IrrE family metallo-endopeptidase -
  SPJ_RS00170 (SPJ_0034) - 27672..27935 (-) 264 WP_000285962.1 type II toxin-antitoxin system RelE/ParE family toxin -
  SPJ_RS00175 (SPJ_0035) - 27935..28168 (-) 234 WP_000156419.1 hypothetical protein -
  SPJ_RS00180 (SPJ_0036) - 28168..28536 (-) 369 WP_000464160.1 helix-turn-helix transcriptional regulator -
  SPJ_RS00185 (SPJ_0038) - 29108..29299 (+) 192 WP_001112859.1 DNA-binding protein -
  SPJ_RS00190 (SPJ_0039) - 29322..29525 (+) 204 WP_001247549.1 hypothetical protein -
  SPJ_RS11340 (SPJ_0040) - 29680..29847 (-) 168 WP_000024181.1 YjzC family protein -
  SPJ_RS00195 (SPJ_0042) - 29867..30232 (+) 366 Protein_29 autolysin -
  SPJ_RS00200 (SPJ_0043) - 30452..30631 (-) 180 WP_001209433.1 hypothetical protein -
  SPJ_RS12935 - 30773..30922 (-) 150 WP_001030863.1 hypothetical protein -
  SPJ_RS00205 (SPJ_0045) - 31227..31670 (+) 444 WP_000701992.1 dUTP diphosphatase -
  SPJ_RS00210 (SPJ_0046) - 31672..32187 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  SPJ_RS00215 (SPJ_0047) radA 32201..33562 (+) 1362 WP_075213698.1 DNA repair protein RadA Machinery gene
  SPJ_RS00220 (SPJ_0048) - 33635..34132 (+) 498 WP_001809263.1 carbonic anhydrase -
  SPJ_RS00225 (SPJ_0049) - 34157..34940 (+) 784 Protein_36 PrsW family glutamic-type intramembrane protease -
  SPJ_RS00235 (SPJ_0051) - 35085..36053 (+) 969 WP_000010157.1 ribose-phosphate diphosphokinase -
  SPJ_RS11345 (SPJ_0052) - 36187..36468 (-) 282 Protein_38 ISL3 family transposase -
  SPJ_RS13130 - 36595..37502 (-) 908 Protein_39 Rpn family recombination-promoting nuclease/putative transposase -
  SPJ_RS00255 (SPJ_0056) polA 37758..40391 (+) 2634 WP_000358437.1 DNA polymerase I -
  SPJ_RS00260 (SPJ_0057) - 40476..40913 (+) 438 WP_000076479.1 CoA-binding protein -
  SPJ_RS00265 (SPJ_0058) - 41376..42386 (-) 1011 WP_000009143.1 YeiH family protein -
  SPJ_RS00270 (SPJ_0059) - 42535..43704 (+) 1170 WP_000366378.1 pyridoxal phosphate-dependent aminotransferase -
  SPJ_RS00275 (SPJ_0060) recO 43701..44471 (+) 771 WP_000616164.1 DNA repair protein RecO -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.91 Da        Isoelectric Point: 6.1868

>NTDB_id=33127 SPJ_RS00215 WP_075213698.1 32201..33562(+) (radA) [Streptococcus pneumoniae JJA]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=33127 SPJ_RS00215 WP_075213698.1 32201..33562(+) (radA) [Streptococcus pneumoniae JJA]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTCAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X2XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment