Detailed information NTG0331068

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EJD96_RS01955
• Genome accession: NZ_CP034395
• Coordinates: 369131..369994 (-)
• Length: 287 a.a.
• NCBI ID: WP_139969535.1
• Uniprot ID: -
• Organism: Herbaspirillum seropedicae strain AU13965
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 364131..374994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJD96_RS01925 (EJD96_01925)	argJ	364788..366023 (+)	1236	WP_013232396.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
EJD96_RS01930 (EJD96_01930)	-	366032..366907 (+)	876	WP_013232397.1	ATP-binding protein	-
EJD96_RS01935 (EJD96_01935)	-	366907..367338 (+)	432	WP_139969531.1	NUDIX domain-containing protein	-
EJD96_RS01940 (EJD96_01940)	yacG	367376..367561 (-)	186	WP_013232399.1	DNA gyrase inhibitor YacG	-
EJD96_RS01945 (EJD96_01945)	zapD	367591..368346 (-)	756	WP_013232400.1	cell division protein ZapD	-
EJD96_RS01950 (EJD96_01950)	coaE	368499..369119 (-)	621	WP_139969533.1	dephospho-CoA kinase	-
EJD96_RS01955 (EJD96_01955)	pilD	369131..369994 (-)	864	WP_139969535.1	prepilin peptidase	Machinery gene
EJD96_RS01960 (EJD96_01960)	-	370046..371293 (-)	1248	WP_139969537.1	type II secretion system F family protein	-
EJD96_RS01965 (EJD96_01965)	pilF	371280..372413 (-)	1134	WP_139969539.1	GspE/PulE family protein	Machinery gene
EJD96_RS01970 (EJD96_01970)	-	372577..373881 (-)	1305	WP_139969541.1	HlyC/CorC family transporter	-
EJD96_RS01980 (EJD96_01980)	infA	374310..374573 (+)	264	WP_013232407.1	translation initiation factor IF-1	-
EJD96_RS01985 (EJD96_01985)	-	374685..374936 (+)	252	WP_244940135.1	hypothetical protein	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 31179.36 Da
• Isoelectric Point: 7.9356

>NTDB_id=331068 EJD96_RS01955 WP_139969535.1 369131..369994(-) (pilD) [Herbaspirillum seropedicae strain AU13965]
MPFLEAYALPGDPILCALAALLGLLVGSFLNVLIHRLPIMMLRESANFVAQERGETPLHHDRYDLWLPSSACPHCATPLP
WRHKLPVLSWLLLRGRCGHCQAAIGLRYPLVELLTAALFAFAAWRFGLGVTGLAVMACTATLVALAFIDAQTMLLPDDLT
LPLMWLGLLVNLTHRLVPLQDAVLGAAAGYGVLWLIFWLFKLITGKDGLGYGDFKLMAALGAWLGWQALPVILLLASAMG
AVLGLVLMAAGRHRRGQAIPFGPCLASAGLLALLQGLDWLWRGLYDY

Nucleotide

• Length: 864 bp

>NTDB_id=331068 EJD96_RS01955 WP_139969535.1 369131..369994(-) (pilD) [Herbaspirillum seropedicae strain AU13965]
ATGCCCTTCCTGGAAGCCTACGCCCTCCCTGGCGACCCCATCCTCTGCGCGCTGGCGGCCCTGCTGGGCTTGCTGGTGGG
CAGTTTCCTCAATGTGCTGATCCACCGCCTGCCCATCATGATGCTGCGCGAAAGCGCCAACTTCGTGGCGCAGGAGCGCG
GGGAAACGCCGCTCCATCACGACCGCTACGACCTCTGGCTGCCCTCATCGGCCTGTCCGCACTGCGCCACCCCTTTGCCC
TGGCGTCACAAGCTGCCGGTACTGAGCTGGCTGCTGCTGCGCGGGCGCTGCGGCCACTGCCAGGCCGCCATCGGCTTGCG
TTATCCATTGGTAGAGCTGTTGACGGCGGCACTGTTCGCCTTCGCAGCCTGGCGCTTCGGCCTGGGCGTCACGGGCCTGG
CCGTGATGGCCTGCACGGCCACGCTGGTCGCGCTGGCCTTCATCGACGCCCAGACCATGCTGCTGCCCGACGACCTGACC
CTGCCGCTGATGTGGCTGGGCCTGCTGGTGAACCTGACGCACCGCCTGGTGCCCCTGCAGGATGCGGTGTTGGGCGCTGC
GGCCGGGTATGGCGTGCTATGGCTGATCTTCTGGCTCTTCAAGCTCATCACCGGCAAGGATGGGCTGGGCTATGGCGACT
TCAAGCTCATGGCTGCGCTGGGCGCCTGGCTGGGCTGGCAGGCGCTGCCGGTGATTTTGCTGCTGGCCTCGGCCATGGGC
GCGGTGCTAGGGTTGGTGCTGATGGCGGCTGGCCGGCATCGCCGTGGCCAGGCCATTCCCTTCGGACCTTGCCTGGCCAG
CGCCGGCCTCTTGGCGCTGTTGCAGGGACTGGACTGGCTCTGGCGAGGACTTTACGACTATTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.309	94.774	0.505
pilD	Vibrio campbellii strain DS40M4	48.387	97.213	0.47
pilD	Neisseria gonorrhoeae MS11	48.551	96.167	0.467
pilD	Acinetobacter baumannii D1279779	43.956	95.122	0.418
pilD	Acinetobacter nosocomialis M2	43.223	95.122	0.411