Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EI693_RS24725 Genome accession   NZ_CP034337
Coordinates   5353612..5354880 (-) Length   422 a.a.
NCBI ID   WP_125466267.1    Uniprot ID   -
Organism   Pseudomonas oryziphila strain 2014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5348612..5359880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EI693_RS24710 (EI693_24710) - 5350273..5351877 (-) 1605 WP_125465908.1 AAA family ATPase -
  EI693_RS24715 (EI693_24715) aroB 5351893..5352990 (-) 1098 WP_125465909.1 3-dehydroquinate synthase -
  EI693_RS24720 (EI693_24720) aroK 5353089..5353607 (-) 519 WP_110996384.1 shikimate kinase AroK -
  EI693_RS24725 (EI693_24725) pilQ 5353612..5354880 (-) 1269 WP_125466267.1 type IV pilus secretin PilQ Machinery gene
  EI693_RS24730 (EI693_24730) - 5354810..5355820 (-) 1011 WP_125465910.1 pilus assembly protein PilP -
  EI693_RS24735 (EI693_24735) - 5355817..5356353 (-) 537 WP_125465911.1 PilN domain-containing protein -
  EI693_RS24740 (EI693_24740) pilM 5356353..5357243 (-) 891 WP_125465912.1 type IV pilus biogenesis protein PilM -
  EI693_RS24745 (EI693_24745) - 5357412..5359862 (+) 2451 WP_394366672.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45938.79 Da        Isoelectric Point: 4.8663

>NTDB_id=330776 EI693_RS24725 WP_125466267.1 5353612..5354880(-) (pilQ) [Pseudomonas oryziphila strain 2014]
MKRWSSGGLMLLLGLRATLATAFEKEPISLNFQDVEVRAVLQVMADYVGINLVASDTVQGRISLRLDDVPWQQALDLVLR
SKGLGRRQEGNVLLVAPVAELAEQSRDAHLEQALEAQLEPLQRELIPVHHARAAELAELLLASLADDGILAGRGSLSVDA
RTNTLVVAQPPERIAELRKLVAQLDVPVRQVMIEARIVEAGVDYEKVLGVRWGGPLYGQNSRLGSELFVDLGVERGTSGL
GVGLLRSDILLDLELSAMEKSGNGEIISQPKVVTADKETARIIKGTEVPYQETTKSGATSISFREASLSLEVTPQITPDG
QVVMEVLVTKDEPDFVNALNNVPPIRKNEVNAKVRVADGQTIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=330776 EI693_RS24725 WP_125466267.1 5353612..5354880(-) (pilQ) [Pseudomonas oryziphila strain 2014]
ATGAAAAGGTGGTCGAGCGGGGGGCTGATGCTCCTGCTGGGCTTGAGGGCGACGCTGGCGACGGCGTTTGAGAAAGAGCC
CATATCGCTGAACTTCCAGGACGTGGAAGTGCGCGCGGTGCTGCAGGTCATGGCTGACTACGTGGGGATCAACCTGGTGG
CCAGCGATACGGTCCAGGGCCGCATCAGCCTGCGCCTTGACGATGTGCCGTGGCAGCAGGCGCTGGATCTGGTGCTGCGC
AGCAAAGGGCTGGGGCGGCGCCAGGAGGGCAATGTGCTGCTGGTCGCGCCGGTTGCCGAACTTGCCGAGCAGTCGCGCGA
TGCCCACCTGGAGCAGGCGCTGGAGGCGCAACTGGAACCCTTGCAGCGCGAGCTGATTCCGGTCCATCACGCCCGGGCTG
CGGAACTGGCCGAGTTGCTGCTGGCCAGCCTTGCCGATGACGGCATCCTGGCCGGGCGCGGCAGCCTCAGCGTGGATGCA
CGGACCAATACCCTGGTGGTGGCCCAGCCGCCAGAGCGGATCGCCGAGCTGCGCAAGCTGGTGGCGCAGCTCGATGTACC
CGTACGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGGCGTCGATTACGAAAAGGTACTGGGCGTGCGTTGGGGAG
GCCCTTTGTATGGGCAGAACAGCCGGTTGGGCAGCGAGCTGTTTGTCGACCTGGGGGTGGAGCGGGGCACGTCGGGGCTG
GGTGTGGGCCTGCTGCGCAGCGACATACTGCTGGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGCAACGGCGAGATCAT
CTCGCAGCCCAAGGTGGTCACTGCCGACAAAGAGACCGCGCGCATCATCAAGGGTACCGAGGTGCCTTACCAGGAGACGA
CCAAGAGTGGTGCCACCTCCATCTCGTTCCGCGAGGCCTCGCTGTCGCTGGAAGTAACGCCGCAGATCACCCCGGATGGC
CAGGTGGTGATGGAGGTGCTGGTGACCAAGGATGAGCCGGATTTCGTCAACGCCCTGAACAATGTGCCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTGCGGGTGGCCGATGGCCAGACCATTGTGATTGGCGGCGTCTACTCGACGTCGCAGAACA
ATGTGGTCGACAAGGTGCCATTTTTGGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.895

100

0.6
  comQ Acinetobacter baylyi ADP1

44.989

100

0.479
  pilQ Acinetobacter baumannii strain A118

41.703

100

0.453
  pilQ Acinetobacter baumannii D1279779

41.703

100

0.453
  pilQ Vibrio campbellii strain DS40M4

42.453

100

0.427
  pilQ Legionella pneumophila strain ERS1305867

43.062

99.052

0.427
  pilQ Legionella pneumophila strain Lp02

43.062

99.052

0.427
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.755

99.763

0.427
  pilQ Vibrio cholerae strain A1552

42.755

99.763

0.427
  pilQ Neisseria gonorrhoeae strain FA1090

37.619

99.526

0.374
  pilQ Neisseria meningitidis MC58

36.667

99.526

0.365
  pilQ Neisseria meningitidis M1080

36.429

99.526

0.363

Multiple sequence alignment