Detailed information NTG0330706

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EJB01_RS21435
• Genome accession: NZ_CP034321
• Coordinates: 4299023..4299823 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 33
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4294023..4304823

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJB01_RS21400 (EJB01_21365)	gspH	4294179..4294694 (+)	516	WP_032435598.1	type II secretion system minor pseudopilin GspH	-
EJB01_RS21405 (EJB01_21370)	gspI	4294691..4295056 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
EJB01_RS21410 (EJB01_21375)	gspJ	4295053..4295649 (+)	597	WP_032435596.1	type II secretion system minor pseudopilin GspJ	-
EJB01_RS21415 (EJB01_21380)	gspK	4295646..4296632 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
EJB01_RS21420 (EJB01_21385)	gspL	4296619..4297812 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
EJB01_RS21425 (EJB01_21390)	-	4297805..4298290 (+)	486	WP_004145896.1	type II secretion system protein M	-
EJB01_RS21430 (EJB01_21395)	-	4298280..4299026 (+)	747	WP_004145897.1	type II secretion system protein N	-
EJB01_RS21435 (EJB01_21400)	pilD	4299023..4299823 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
EJB01_RS21440 (EJB01_21405)	hrpB	4299871..4302300 (-)	2430	WP_032435594.1	ATP-dependent helicase HrpB	-
EJB01_RS21445 (EJB01_21410)	thpR	4302373..4302909 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
EJB01_RS21450 (EJB01_21415)	sfsA	4302909..4303625 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
EJB01_RS21455 (EJB01_21420)	dksA	4303788..4304243 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=330706 EJB01_RS21435 WP_002888947.1 4299023..4299823(+) (pilD) [Klebsiella pneumoniae strain 33]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=330706 EJB01_RS21435 WP_002888947.1 4299023..4299823(+) (pilD) [Klebsiella pneumoniae strain 33]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGGCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425