Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EHC69_RS15475 Genome accession   NZ_CP034298
Coordinates   3107819..3108835 (-) Length   338 a.a.
NCBI ID   WP_005480475.1    Uniprot ID   A0A0L7V0I6
Organism   Vibrio parahaemolyticus strain 20160303005-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3102819..3113835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHC69_RS15450 (EHC69_15630) aroK 3103679..3104197 (-) 519 WP_005381319.1 shikimate kinase AroK -
  EHC69_RS15455 (EHC69_15635) pilQ 3104386..3106134 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  EHC69_RS15460 (EHC69_15640) pilP 3106166..3106681 (-) 516 WP_069538154.1 pilus assembly protein PilP Machinery gene
  EHC69_RS15465 (EHC69_15645) pilO 3106671..3107264 (-) 594 WP_114867891.1 type 4a pilus biogenesis protein PilO Machinery gene
  EHC69_RS15470 (EHC69_15650) pilN 3107257..3107835 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  EHC69_RS15475 (EHC69_15655) pilM 3107819..3108835 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene
  EHC69_RS15480 (EHC69_15660) - 3108978..3111548 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  EHC69_RS15485 (EHC69_15665) oxyR 3111604..3112512 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  EHC69_RS15490 (EHC69_15670) - 3112683..3113411 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37776.31 Da        Isoelectric Point: 6.8323

>NTDB_id=330554 EHC69_RS15475 WP_005480475.1 3107819..3108835(-) (pilM) [Vibrio parahaemolyticus strain 20160303005-1]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=330554 EHC69_RS15475 WP_005480475.1 3107819..3108835(-) (pilM) [Vibrio parahaemolyticus strain 20160303005-1]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L7V0I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment