Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EHC70_RS01860 Genome accession   NZ_CP034285
Coordinates   362896..363912 (+) Length   338 a.a.
NCBI ID   WP_017449463.1    Uniprot ID   A0A7Y0SFN2
Organism   Vibrio parahaemolyticus strain 20140624012-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 357896..368912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHC70_RS01845 (EHC70_01880) - 358320..359048 (-) 729 WP_015297397.1 redoxin family protein -
  EHC70_RS01850 (EHC70_01885) oxyR 359219..360127 (+) 909 WP_159404022.1 DNA-binding transcriptional regulator OxyR -
  EHC70_RS01855 (EHC70_01890) - 360183..362753 (-) 2571 WP_005480476.1 penicillin-binding protein 1A -
  EHC70_RS01860 (EHC70_01895) pilM 362896..363912 (+) 1017 WP_017449463.1 type IV pilus biogenesis protein PilM Machinery gene
  EHC70_RS01865 (EHC70_01900) pilN 363896..364474 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  EHC70_RS01870 (EHC70_01905) pilO 364467..365060 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  EHC70_RS01875 (EHC70_01910) pilP 365050..365565 (+) 516 WP_159404023.1 pilus assembly protein PilP Machinery gene
  EHC70_RS01880 (EHC70_01915) pilQ 365597..367345 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  EHC70_RS01885 (EHC70_01920) aroK 367534..368052 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.26 Da        Isoelectric Point: 6.8322

>NTDB_id=330391 EHC70_RS01860 WP_017449463.1 362896..363912(+) (pilM) [Vibrio parahaemolyticus strain 20140624012-1]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=330391 EHC70_RS01860 WP_017449463.1 362896..363912(+) (pilM) [Vibrio parahaemolyticus strain 20140624012-1]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGATTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0SFN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment