Detailed information NTG0330342

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EIP93_RS15425
• Genome accession: NZ_CP034276
• Coordinates: 3395608..3396453 (-)
• Length: 281 a.a.
• NCBI ID: WP_107333189.1
• Uniprot ID: -
• Organism: Pectobacterium versatile strain 14A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3390608..3401453

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EIP93_RS15400 (EIP93_15445)	-	3390784..3391554 (-)	771	WP_125232933.1	ABC transporter ATP-binding protein	-
EIP93_RS15405 (EIP93_15450)	-	3391556..3392593 (-)	1038	WP_125232934.1	FecCD family ABC transporter permease	-
EIP93_RS15410 (EIP93_15455)	-	3392590..3393654 (-)	1065	WP_125232935.1	ABC transporter substrate-binding protein	-
EIP93_RS15415 (EIP93_15460)	-	3394006..3395031 (+)	1026	WP_103862229.1	LLM class flavin-dependent oxidoreductase	-
EIP93_RS15420 (EIP93_15465)	ypdK	3395388..3395462 (+)	75	WP_099606229.1	membrane protein YpdK	-
EIP93_RS15425 (EIP93_15470)	pilD	3395608..3396453 (-)	846	WP_107333189.1	prepilin peptidase	Machinery gene
EIP93_RS15430 (EIP93_15475)	-	3396543..3397289 (-)	747	WP_107333190.1	type II secretion system protein N	-
EIP93_RS15435 (EIP93_15480)	gspM	3397279..3397773 (-)	495	WP_103862232.1	type II secretion system protein GspM	-
EIP93_RS15440 (EIP93_15485)	gspL	3397770..3399047 (-)	1278	WP_107333191.1	type II secretion system protein GspL	-
EIP93_RS15445 (EIP93_15490)	gspK	3399044..3400030 (-)	987	WP_010302966.1	type II secretion system minor pseudopilin GspK	-
EIP93_RS15450 (EIP93_15495)	gspJ	3400027..3400677 (-)	651	WP_103940956.1	type II secretion system minor pseudopilin GspJ	-
EIP93_RS15455 (EIP93_15500)	gspI	3400677..3401042 (-)	366	WP_107333192.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31322.16 Da
• Isoelectric Point: 7.0813

>NTDB_id=330342 EIP93_RS15425 WP_107333189.1 3395608..3396453(-) (pilD) [Pectobacterium versatile strain 14A]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDRDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSAQYPLVEVITMLAFLAAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=330342 EIP93_RS15425 WP_107333189.1 3395608..3396453(-) (pilD) [Pectobacterium versatile strain 14A]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCGTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAACAGGCTATT
GCGGTCAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGTCATCAATCGGTCTCTGC
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATCCTGTTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTTTCATTGAACGATGCCGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCCACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTTTGGTGGCAGCGTTGAGC
GGTCTGGTCGTTACTCTCGTCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGTTAGCTTTTGGCCCCTGGCTGGC
AATGGGTGGCGTGTTTGGTGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.554	96.441	0.488
pilD	Vibrio campbellii strain DS40M4	45.556	96.085	0.438
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Acinetobacter baumannii D1279779	42.491	97.153	0.413
pilD	Acinetobacter nosocomialis M2	42.125	97.153	0.409