Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FPV29_RS18740 Genome accession   NZ_CP041919
Coordinates   3859547..3859987 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain Ec40743     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3854547..3864987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPV29_RS18720 (FPV29_18720) aroP 3855545..3856918 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FPV29_RS18725 (FPV29_18725) ampE 3856961..3857815 (-) 855 WP_000171999.1 beta-lactamase regulator AmpE -
  FPV29_RS18730 (FPV29_18730) ampD 3857812..3858363 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FPV29_RS18735 (FPV29_18735) nadC 3858451..3859344 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FPV29_RS18740 (FPV29_18740) pilA 3859547..3859987 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FPV29_RS18745 (FPV29_18745) pilB 3859997..3861382 (+) 1386 WP_023156327.1 type II secretion system protein GspE Machinery gene
  FPV29_RS18750 (FPV29_18750) hofC 3861372..3862574 (+) 1203 WP_000157234.1 protein transport protein HofC -
  FPV29_RS18755 (FPV29_18755) guaC 3862609..3863652 (-) 1044 WP_001217338.1 GMP reductase -
  FPV29_RS24895 - 3863808..3863852 (-) 45 WP_120795372.1 protein YacM -
  FPV29_RS18760 (FPV29_18760) coaE 3863877..3864497 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=328968 FPV29_RS18740 WP_000360895.1 3859547..3859987(+) (pilA) [Escherichia coli strain Ec40743]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=328968 FPV29_RS18740 WP_000360895.1 3859547..3859987(+) (pilA) [Escherichia coli strain Ec40743]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363