Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   EGT90_RS10120 Genome accession   NZ_CP034099
Coordinates   1932002..1932889 (+) Length   295 a.a.
NCBI ID   WP_127429534.1    Uniprot ID   -
Organism   Limosilactobacillus fermentum strain LMT2-75     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1927002..1937889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT90_RS10095 - 1927234..1928619 (+) 1386 WP_057194737.1 amino acid permease -
  EGT90_RS10100 - 1928696..1929672 (-) 977 Protein_1923 IS30 family transposase -
  EGT90_RS10110 - 1929862..1930557 (+) 696 WP_262337278.1 hypothetical protein -
  EGT90_RS10115 - 1930557..1931927 (+) 1371 WP_057195143.1 C69 family dipeptidase -
  EGT90_RS10120 endA 1932002..1932889 (+) 888 WP_127429534.1 DNA/RNA non-specific endonuclease Machinery gene
  EGT90_RS10125 - 1932980..1933867 (-) 888 WP_014562426.1 3-hydroxyacyl-CoA dehydrogenase -
  EGT90_RS10130 - 1934254..1936602 (+) 2349 WP_003684744.1 hypothetical protein -
  EGT90_RS10135 - 1936741..1937598 (+) 858 WP_035423509.1 alpha/beta hydrolase -
  EGT90_RS12285 - 1937606..1937782 (+) 177 WP_003682971.1 hypothetical protein -

Sequence

Protein


Download         Length: 295 a.a.        Molecular weight: 32097.92 Da        Isoelectric Point: 10.7907

>NTDB_id=328969 EGT90_RS10120 WP_127429534.1 1932002..1932889(+) (endA) [Limosilactobacillus fermentum strain LMT2-75]
MKRRRNKKQGWRWLIAFVILFALASGIPGKASSTTITSSPSAQTLVAKVNSLVENLSYRSRSSSTSGSSTMVTPSKQKAE
AVLTANVKQQLGSSITWNGDGAFIINNNKTKLNAKINNAPYAVDRLDSQGRAWQADAWLNKTTRQYKNRSETGNGATSWK
PVGFMQLTNLKNGPTHAYDRGHLLGYALVGGLSGFNASESNPKNIATQTAWANEARSTDSTGQNYYEGLVRKALDRGKQV
RYRVTDIYDGNNLVPSGAHIEAKSKDGSLQFNVFVPNVQNNITINYATGKATATN

Nucleotide


Download         Length: 888 bp        

>NTDB_id=328969 EGT90_RS10120 WP_127429534.1 1932002..1932889(+) (endA) [Limosilactobacillus fermentum strain LMT2-75]
ATGAAACGCCGACGTAACAAGAAGCAGGGCTGGCGCTGGTTAATTGCCTTCGTCATCCTCTTCGCTCTTGCCTCGGGTAT
CCCGGGCAAGGCATCTTCTACGACCATCACTAGTTCACCGAGTGCCCAAACCCTGGTTGCCAAGGTCAACTCCCTGGTGG
AAAACCTGAGCTACCGGTCGCGCTCATCGTCAACCAGCGGCTCGAGCACGATGGTCACCCCGAGCAAGCAAAAGGCCGAG
GCGGTTTTAACCGCTAACGTTAAGCAACAACTGGGGAGTTCGATCACTTGGAATGGCGATGGGGCCTTCATTATCAACAA
CAATAAGACCAAGCTCAACGCCAAGATCAACAACGCGCCCTACGCCGTTGACCGGCTCGACAGCCAGGGGCGTGCTTGGC
AGGCCGATGCCTGGTTAAACAAAACGACGCGCCAATACAAGAATCGTTCCGAAACCGGGAACGGCGCCACTAGTTGGAAA
CCGGTCGGCTTTATGCAACTGACCAACTTAAAGAACGGCCCGACCCACGCTTATGACCGGGGGCACCTACTCGGCTACGC
CTTGGTCGGGGGGCTCTCCGGTTTCAACGCTTCGGAGTCCAACCCGAAAAACATCGCCACCCAAACCGCTTGGGCTAACG
AAGCCCGGTCAACCGATTCAACCGGGCAAAACTACTACGAAGGCCTAGTTCGCAAGGCCCTCGACCGGGGTAAGCAAGTC
CGCTACCGGGTAACCGACATTTATGACGGTAACAACCTAGTTCCGTCCGGCGCCCACATTGAAGCCAAGTCAAAGGACGG
GAGCCTCCAGTTCAACGTCTTTGTTCCGAACGTTCAAAATAACATTACGATCAATTACGCCACTGGGAAAGCCACCGCTA
CTAACTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(115-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

57.028

84.407

0.481
  endA Streptococcus pneumoniae D39

57.028

84.407

0.481
  endA Streptococcus pneumoniae R6

57.028

84.407

0.481
  endA Streptococcus pneumoniae TIGR4

57.028

84.407

0.481

Multiple sequence alignment