Detailed information NTG0328876

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: EHF38_RS01675
• Genome accession: NZ_CP034092
• Coordinates: 352119..352544 (+)
• Length: 141 a.a.
• NCBI ID: WP_000788337.1
• Uniprot ID: A0A009SG52
• Organism: Acinetobacter baumannii strain A52
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	350009..351217	352119..352544	flank	902

Gene organization within MGE regions

Location: 350009..352544

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EHF38_RS01660 (EHF38_01660)	-	350009..351217 (-)	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-
EHF38_RS01665 (EHF38_01665)	pilY1	351307..351627 (+)	321	WP_128385708.1	hypothetical protein	Machinery gene
EHF38_RS01670 (EHF38_01670)	pilY2	351640..352122 (+)	483	WP_001046421.1	type IV pilin protein	Machinery gene
EHF38_RS01675 (EHF38_01675)	pilE	352119..352544 (+)	426	WP_000788337.1	type IV pilin protein	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 15163.36 Da
• Isoelectric Point: 7.8144

>NTDB_id=328876 EHF38_RS01675 WP_000788337.1 352119..352544(+) (pilE) [Acinetobacter baumannii strain A52]
MKNGFSLIEIMVVVAIVAILAAIATPSYLQYLRKGHRTAVQSEMMNIAQTLESQKIVNNRYPSDATIQSIYGSNVSPIQG
QALYKLAFATLNDSTWVLTATPISTSSQAGDGIICLNDQGQKFWAKGATVCALSASSSWTE

Nucleotide

• Length: 426 bp

>NTDB_id=328876 EHF38_RS01675 WP_000788337.1 352119..352544(+) (pilE) [Acinetobacter baumannii strain A52]
GTGAAGAATGGTTTTTCTTTAATTGAAATTATGGTTGTGGTAGCAATAGTTGCAATTTTAGCGGCTATAGCCACCCCTTC
ATATTTACAATATTTGCGTAAAGGACATCGTACTGCTGTTCAATCCGAAATGATGAATATTGCGCAGACATTAGAATCTC
AAAAAATAGTAAATAACCGTTATCCTTCGGATGCGACCATACAGTCGATTTATGGTTCTAATGTGAGTCCTATACAAGGT
CAGGCTCTATATAAGTTAGCTTTTGCAACTCTAAATGATTCAACTTGGGTACTTACTGCAACACCTATTTCTACTAGCTC
ACAAGCAGGTGATGGAATTATTTGTTTAAATGACCAAGGGCAAAAATTTTGGGCAAAAGGTGCCACAGTTTGTGCACTAT
CTGCTTCATCGAGTTGGACCGAGTAA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (26-115)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-24)

3D structure

• AlphaFold DB: A0A009SG52

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Acinetobacter baumannii D1279779	90.78	100	0.908
comF	Acinetobacter baylyi ADP1	50	99.291	0.496