Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   EG219_RS16865 Genome accession   NZ_CP034037
Coordinates   3388940..3389059 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus velezensis strain BCSo1     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3383940..3394059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG219_RS16840 (EG219_16840) - 3384318..3385034 (-) 717 WP_007407631.1 Crp/Fnr family transcriptional regulator -
  EG219_RS16845 (EG219_16845) narK 3385132..3386322 (-) 1191 WP_007407632.1 nitrate transporter NarK -
  EG219_RS16850 (EG219_16850) argS 3386455..3388125 (-) 1671 WP_007407633.1 arginine--tRNA ligase -
  EG219_RS16855 (EG219_16855) - 3388122..3388220 (-) 99 Protein_3249 DUF1934 domain-containing protein -
  EG219_RS16860 (EG219_16860) - 3388387..3388881 (+) 495 Protein_3250 aspartate phosphatase -
  EG219_RS16865 (EG219_16865) phrF 3388940..3389059 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  EG219_RS16870 (EG219_16870) - 3389257..3389460 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  EG219_RS16875 (EG219_16875) - 3389474..3389677 (+) 204 WP_007407641.1 hypothetical protein -
  EG219_RS16880 (EG219_16880) - 3389712..3389897 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  EG219_RS16885 (EG219_16885) - 3390083..3390364 (+) 282 WP_007407643.1 hypothetical protein -
  EG219_RS16890 (EG219_16890) - 3390408..3390920 (+) 513 WP_007407644.1 stage II sporulation protein M -
  EG219_RS16900 (EG219_16900) - 3391511..3391729 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  EG219_RS16905 (EG219_16905) speB 3391897..3392769 (-) 873 WP_007407646.1 agmatinase -
  EG219_RS16910 (EG219_16910) speE 3392828..3393658 (-) 831 WP_003151052.1 spermidine synthase -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=328572 EG219_RS16865 WP_007407639.1 3388940..3389059(+) (phrF) [Bacillus velezensis strain BCSo1]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=328572 EG219_RS16865 WP_007407639.1 3388940..3389059(+) (phrF) [Bacillus velezensis strain BCSo1]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513

  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462


Multiple sequence alignment