Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EG219_RS11580 Genome accession   NZ_CP034037
Coordinates   2374060..2374674 (-) Length   204 a.a.
NCBI ID   WP_007408263.1    Uniprot ID   -
Organism   Bacillus velezensis strain BCSo1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2369060..2379674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG219_RS11550 (EG219_11550) rpsT 2369364..2369630 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  EG219_RS11555 (EG219_11555) holA 2369647..2370688 (-) 1042 Protein_2233 DNA polymerase III subunit delta -
  EG219_RS11560 (EG219_11560) - 2370746..2370879 (-) 134 Protein_2234 hypothetical protein -
  EG219_RS11565 (EG219_11565) - 2370920..2371054 (+) 135 WP_003152870.1 YqzM family protein -
  EG219_RS11570 (EG219_11570) comEC 2371073..2373424 (-) 2352 WP_007408265.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EG219_RS11575 (EG219_11575) - 2373425..2373994 (-) 570 WP_007408264.1 ComE operon protein 2 -
  EG219_RS11580 (EG219_11580) comEA 2374060..2374674 (-) 615 WP_007408263.1 helix-hairpin-helix domain-containing protein Machinery gene
  EG219_RS11585 (EG219_11585) comER 2374733..2375554 (+) 822 WP_007408262.1 late competence protein ComER -
  EG219_RS11590 (EG219_11590) - 2375623..2376360 (-) 738 WP_007408261.1 class I SAM-dependent methyltransferase -
  EG219_RS11595 (EG219_11595) rsfS 2376357..2376713 (-) 357 WP_007408260.1 ribosome silencing factor -
  EG219_RS11600 (EG219_11600) yqeK 2376731..2377291 (-) 561 WP_007408259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EG219_RS11605 (EG219_11605) - 2377281..2377850 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  EG219_RS11610 (EG219_11610) yhbY 2377861..2378151 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  EG219_RS11615 (EG219_11615) aroE 2378145..2378987 (-) 843 WP_007408256.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22223.39 Da        Isoelectric Point: 6.4940

>NTDB_id=328552 EG219_RS11580 WP_007408263.1 2374060..2374674(-) (comEA) [Bacillus velezensis strain BCSo1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=328552 EG219_RS11580 WP_007408263.1 2374060..2374674(-) (comEA) [Bacillus velezensis strain BCSo1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.333

94.118

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363

Multiple sequence alignment