Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EG219_RS01650 Genome accession   NZ_CP034037
Coordinates   305251..305688 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain BCSo1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 300251..310688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG219_RS01615 (EG219_01615) - 300832..300984 (-) 153 Protein_296 molybdopterin dinucleotide binding domain-containing protein -
  EG219_RS01620 (EG219_01620) - 301108..302310 (+) 1203 WP_007409360.1 GTP-binding protein -
  EG219_RS01625 (EG219_01625) - 302375..303511 (+) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -
  EG219_RS01630 (EG219_01630) - 303526..303960 (+) 435 WP_007409358.1 RDD family protein -
  EG219_RS01635 (EG219_01635) - 304032..304355 (+) 324 WP_007409357.1 YckD family protein -
  EG219_RS18705 - 304459..304569 (+) 111 Protein_301 family 1 glycosylhydrolase -
  EG219_RS18710 - 304591..304791 (+) 201 Protein_302 family 1 glycosylhydrolase -
  EG219_RS01645 (EG219_01645) nin/comJ 304832..305230 (-) 399 WP_007409355.1 competence protein ComJ Regulator
  EG219_RS01650 (EG219_01650) nucA/comI 305251..305688 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  EG219_RS01655 (EG219_01655) hxlB 306045..306602 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  EG219_RS01660 (EG219_01660) hxlA 306599..307234 (-) 636 WP_007409353.1 3-hexulose-6-phosphate synthase -
  EG219_RS01665 (EG219_01665) - 307465..307827 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=328510 EG219_RS01650 WP_003156588.1 305251..305688(-) (nucA/comI) [Bacillus velezensis strain BCSo1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=328510 EG219_RS01650 WP_003156588.1 305251..305688(-) (nucA/comI) [Bacillus velezensis strain BCSo1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment