Detailed information NTG0328133

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: FOG69_RS00945
• Genome accession: NZ_CP041693
• Coordinates: 168432..169553 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens strain H
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 163432..174553

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOG69_RS00925 (FOG69_00925)	-	163752..164747 (-)	996	WP_014469697.1	DUF5105 domain-containing protein	-
FOG69_RS00930 (FOG69_00930)	-	164865..166262 (+)	1398	WP_014469698.1	YcdB/YcdC domain-containing protein	-
FOG69_RS00935 (FOG69_00935)	-	166464..167822 (+)	1359	WP_014469699.1	YcdB/YcdC domain-containing protein	-
FOG69_RS00940 (FOG69_00940)	-	167819..168322 (-)	504	WP_014469700.1	M15 family metallopeptidase	-
FOG69_RS00945 (FOG69_00945)	rapF	168432..169553 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
FOG69_RS00950 (FOG69_00950)	-	169667..170446 (+)	780	WP_013350920.1	glucose 1-dehydrogenase	-
FOG69_RS00955 (FOG69_00955)	-	170530..172209 (+)	1680	WP_013350921.1	alpha-glucosidase	-
FOG69_RS00960 (FOG69_00960)	-	172194..172688 (-)	495	WP_013350922.1	DUF4188 domain-containing protein	-
FOG69_RS00965 (FOG69_00965)	-	172689..173234 (-)	546	WP_013350923.1	PadR family transcriptional regulator	-
FOG69_RS00970 (FOG69_00970)	-	173443..174402 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=328133 FOG69_RS00945 WP_013350919.1 168432..169553(+) (rapF) [Bacillus amyloliquefaciens strain H]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=328133 FOG69_RS00945 WP_013350919.1 168432..169553(+) (rapF) [Bacillus amyloliquefaciens strain H]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAACCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTATGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402