Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   EGH15_RS02740 Genome accession   NZ_CP034016
Coordinates   451064..451672 (+) Length   202 a.a.
NCBI ID   WP_047926669.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain FQ84     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 452214..502365 451064..451672 flank 542


Gene organization within MGE regions


Location: 451064..502365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGH15_RS02740 (EGH15_02740) pilK 451064..451672 (+) 609 WP_047926669.1 pilus assembly protein Machinery gene
  EGH15_RS02745 (EGH15_02745) pilL 451674..452144 (+) 471 WP_169577278.1 PilX family type IV pilin Machinery gene
  EGH15_RS02750 (EGH15_02750) - 452214..452522 (-) 309 WP_010951048.1 AzlD family protein -
  EGH15_RS02755 (EGH15_02755) - 452519..453228 (-) 710 Protein_456 AzlC family ABC transporter permease -
  EGH15_RS02760 (EGH15_02760) dut 453394..453846 (+) 453 WP_003690909.1 dUTP diphosphatase -
  EGH15_RS02765 (EGH15_02765) dapC 453924..455111 (+) 1188 WP_003701073.1 succinyldiaminopimelate transaminase -
  EGH15_RS02770 (EGH15_02770) yaaA 455267..456046 (+) 780 WP_003687925.1 peroxide stress protein YaaA -
  EGH15_RS02785 (EGH15_02785) - 456577..457779 (+) 1203 WP_071198138.1 integrase arm-type DNA-binding domain-containing protein -
  EGH15_RS02795 (EGH15_02795) - 458135..458404 (-) 270 WP_003687928.1 hypothetical protein -
  EGH15_RS02800 (EGH15_02800) - 458599..459282 (-) 684 WP_003687929.1 DUF2786 domain-containing protein -
  EGH15_RS14560 - 459596..459829 (-) 234 Protein_463 hypothetical protein -
  EGH15_RS02810 (EGH15_02810) - 459940..460155 (-) 216 WP_003691538.1 hypothetical protein -
  EGH15_RS02815 (EGH15_02815) - 460207..460698 (-) 492 WP_010359966.1 siphovirus Gp157 family protein -
  EGH15_RS02820 (EGH15_02820) - 460695..460877 (-) 183 WP_003691535.1 hypothetical protein -
  EGH15_RS02825 (EGH15_02825) - 461017..461703 (-) 687 WP_012503754.1 hypothetical protein -
  EGH15_RS02830 (EGH15_02830) - 461772..461933 (-) 162 WP_003702497.1 hypothetical protein -
  EGH15_RS02835 (EGH15_02835) - 461930..462205 (-) 276 WP_064661611.1 hypothetical protein -
  EGH15_RS02840 (EGH15_02840) - 462358..462690 (-) 333 WP_003687946.1 hypothetical protein -
  EGH15_RS02845 (EGH15_02845) - 462831..463118 (-) 288 WP_082278089.1 hypothetical protein -
  EGH15_RS02850 (EGH15_02850) - 463115..463591 (-) 477 WP_012504141.1 hypothetical protein -
  EGH15_RS02855 (EGH15_02855) - 463624..463824 (-) 201 WP_048654497.1 hypothetical protein -
  EGH15_RS02860 (EGH15_02860) - 464022..464435 (-) 414 WP_003687963.1 hypothetical protein -
  EGH15_RS02865 (EGH15_02865) - 464432..464893 (-) 462 WP_003687965.1 helix-turn-helix transcriptional regulator -
  EGH15_RS02870 (EGH15_02870) - 464910..465347 (-) 438 WP_003687967.1 hypothetical protein -
  EGH15_RS02875 (EGH15_02875) - 465460..466176 (-) 717 WP_003687969.1 LexA family transcriptional regulator -
  EGH15_RS02880 (EGH15_02880) - 466245..466481 (+) 237 WP_003687971.1 Cro/CI family transcriptional regulator -
  EGH15_RS02885 (EGH15_02885) - 466561..466716 (+) 156 WP_003691446.1 hypothetical protein -
  EGH15_RS02890 (EGH15_02890) - 466693..466881 (-) 189 WP_003706568.1 hypothetical protein -
  EGH15_RS02895 (EGH15_02895) - 467054..467281 (+) 228 WP_047949281.1 helix-turn-helix domain-containing protein -
  EGH15_RS02900 (EGH15_02900) - 467278..468243 (+) 966 WP_124693283.1 helix-turn-helix domain-containing protein -
  EGH15_RS02905 (EGH15_02905) - 468258..469040 (+) 783 WP_025456432.1 ATP-binding protein -
  EGH15_RS02910 (EGH15_02910) - 469033..469263 (+) 231 WP_124693284.1 hypothetical protein -
  EGH15_RS02915 (EGH15_02915) - 469354..469848 (+) 495 WP_003691434.1 DUF3310 domain-containing protein -
  EGH15_RS02920 (EGH15_02920) - 470025..470174 (+) 150 WP_003692854.1 hypothetical protein -
  EGH15_RS14075 - 470203..470484 (+) 282 WP_003689109.1 hypothetical protein -
  EGH15_RS02925 (EGH15_02925) - 470475..470855 (+) 381 WP_235220619.1 RusA family crossover junction endodeoxyribonuclease -
  EGH15_RS14415 - 470872..471000 (-) 129 WP_012503747.1 hypothetical protein -
  EGH15_RS02935 (EGH15_02935) - 471122..471529 (+) 408 WP_003691430.1 hypothetical protein -
  EGH15_RS02940 (EGH15_02940) - 471620..472039 (+) 420 WP_229690590.1 type I restriction endonuclease -
  EGH15_RS02945 (EGH15_02945) - 472320..473189 (+) 870 WP_124693286.1 BRO family protein -
  EGH15_RS02950 (EGH15_02950) - 473482..473931 (+) 450 WP_003689093.1 hypothetical protein -
  EGH15_RS02955 (EGH15_02955) terL 473993..475414 (+) 1422 WP_003689090.1 phage terminase large subunit -
  EGH15_RS02960 (EGH15_02960) - 475411..477558 (+) 2148 WP_003691423.1 phage portal protein -
  EGH15_RS02965 (EGH15_02965) - 477626..478783 (+) 1158 WP_010360058.1 HK97 family phage prohead protease -
  EGH15_RS02970 (EGH15_02970) - 478824..480323 (+) 1500 WP_033910832.1 hypothetical protein -
  EGH15_RS13720 (EGH15_02975) - 480330..480692 (+) 363 WP_003689082.1 hypothetical protein -
  EGH15_RS02980 (EGH15_02980) - 480695..481225 (+) 531 WP_003689080.1 head-tail connector protein -
  EGH15_RS02985 (EGH15_02985) - 481225..481707 (+) 483 WP_003703855.1 HK97 gp10 family phage protein -
  EGH15_RS02990 (EGH15_02990) - 481704..482132 (+) 429 WP_003697213.1 hypothetical protein -
  EGH15_RS02995 (EGH15_02995) - 482158..482931 (+) 774 WP_003692869.1 hypothetical protein -
  EGH15_RS03000 (EGH15_03000) - 482992..483321 (+) 330 WP_003692871.1 hypothetical protein -
  EGH15_RS03005 (EGH15_03005) - 483333..483599 (+) 267 WP_003689070.1 hypothetical protein -
  EGH15_RS03010 (EGH15_03010) - 483599..484198 (+) 600 WP_003692874.1 DUF2460 domain-containing protein -
  EGH15_RS03015 (EGH15_03015) - 484195..485049 (+) 855 WP_003698614.1 DUF2163 domain-containing protein -
  EGH15_RS03020 (EGH15_03020) - 485051..485482 (+) 432 WP_003689064.1 NlpC/P60 family protein -
  EGH15_RS03025 (EGH15_03025) - 485510..485788 (-) 279 WP_003689062.1 XRE family transcriptional regulator -
  EGH15_RS03030 (EGH15_03030) - 486028..490173 (+) 4146 WP_071198284.1 phage tail protein -
  EGH15_RS03035 (EGH15_03035) - 490285..490590 (+) 306 WP_003689058.1 hypothetical protein -
  EGH15_RS03040 (EGH15_03040) - 490661..491131 (+) 471 WP_025455988.1 hypothetical protein -
  EGH15_RS03045 (EGH15_03045) - 491132..491464 (+) 333 WP_003691408.1 hypothetical protein -
  EGH15_RS03050 (EGH15_03050) - 491592..491825 (-) 234 WP_003692884.1 hypothetical protein -
  EGH15_RS03055 (EGH15_03055) - 491833..492180 (-) 348 WP_003689051.1 type II toxin-antitoxin system PemK/MazF family toxin -
  EGH15_RS03060 (EGH15_03060) - 492180..492416 (-) 237 WP_003689049.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  EGH15_RS14420 - 492456..492581 (+) 126 WP_255294325.1 hypothetical protein -
  EGH15_RS03070 (EGH15_03070) - 492623..493162 (+) 540 WP_003691405.1 TIGR02594 family protein -
  EGH15_RS03075 (EGH15_03075) - 493163..493507 (+) 345 WP_003695464.1 hypothetical protein -
  EGH15_RS13330 - 493491..493664 (+) 174 WP_165865812.1 hypothetical protein -
  EGH15_RS03080 (EGH15_03080) - 493976..494125 (+) 150 WP_003691402.1 hypothetical protein -
  EGH15_RS03085 (EGH15_03085) - 494155..497199 (+) 3045 WP_082298937.1 tape measure protein -
  EGH15_RS03090 (EGH15_03090) - 497259..497738 (-) 480 WP_002241413.1 DUF4760 domain-containing protein -
  EGH15_RS03100 (EGH15_03100) - 498080..499069 (-) 990 WP_003689040.1 site-specific integrase -
  EGH15_RS03110 (EGH15_03110) purM 499758..500792 (+) 1035 WP_003689038.1 phosphoribosylformylglycinamidine cyclo-ligase -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 22152.01 Da        Isoelectric Point: 5.0966

>NTDB_id=328040 EGH15_RS02740 WP_047926669.1 451064..451672(+) (pilK) [Neisseria gonorrhoeae strain FQ84]
MRKQNALTGIPTSEGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYTA
DSKVTFSENCENGLCTAVNVRTNDANEETFDNIVVKGKPTVEAVKRPCPAKSGKNSAGLCIDNQGVEYEKGTGNVSKMPR
YIIEYLGEKNNQNIYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=328040 EGH15_RS02740 WP_047926669.1 451064..451672(+) (pilK) [Neisseria gonorrhoeae strain FQ84]
ATGCGCAAACAGAACGCTTTGACAGGAATCCCGACTTCTGAGGGACAGAGGGGGTTCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGAGGGAAGGCGAATTTCAGGTTTTGGATTTGGAATATACTGCG
GATAGTAAGGTTACATTTAGCGAAAACTGTGAAAACGGCCTGTGTACCGCAGTGAATGTACGGACAAATGATGCTAATGA
AGAGACTTTTGACAATATCGTGGTGAAAGGCAAGCCTACCGTTGAGGCCGTGAAGCGTCCTTGCCCTGCAAAGTCTGGCA
AAAATTCTGCCGGCCTGTGCATTGACAATCAGGGAGTGGAATATGAGAAAGGTACGGGAAACGTCAGCAAAATGCCGCGT
TATATTATCGAATATTTGGGCGAGAAGAATAACCAAAATATTTACAGGGTTACTGCCAAGGCTTGGGGTAAGAATGCCAA
TACCGTGGTCGTCCTGCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterproScan.

PilX N-terminal (PilX_N)

(17-67)

Type IV pilus assembly protein PilX C-term (PilX)

(92-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

93.596

100

0.941

Multiple sequence alignment