Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CTN_RS00445 Genome accession   NC_011978
Coordinates   87267..88151 (+) Length   294 a.a.
NCBI ID   WP_012644981.1    Uniprot ID   B9KB75
Organism   Thermotoga neapolitana DSM 4359     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 82267..93151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTN_RS00430 (CTN_0092) lnt 83092..84528 (-) 1437 WP_041437364.1 apolipoprotein N-acyltransferase -
  CTN_RS00435 (CTN_0093) - 84521..85672 (-) 1152 WP_038066311.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  CTN_RS00440 (CTN_0094) - 85769..87151 (-) 1383 WP_012644980.1 DegQ family serine endoprotease -
  CTN_RS00445 (CTN_0095) pilA 87267..88151 (+) 885 WP_012644981.1 signal recognition particle-docking protein FtsY Machinery gene
  CTN_RS00450 (CTN_0096) - 88148..88801 (+) 654 WP_012644982.1 DUF2225 domain-containing protein -
  CTN_RS00455 (CTN_0097) - 88777..90441 (+) 1665 WP_012644983.1 peptidoglycan DD-metalloendopeptidase family protein -
  CTN_RS00460 (CTN_0098) - 90452..90859 (+) 408 WP_231555936.1 RrF2 family transcriptional regulator -
  CTN_RS00465 (CTN_0099) - 90878..91072 (-) 195 WP_012644985.1 hypothetical protein -
  CTN_RS00470 (CTN_0100) sfsA 91076..91744 (-) 669 WP_012644986.1 DNA/RNA nuclease SfsA -
  CTN_RS00475 (CTN_0101) - 91741..92220 (-) 480 WP_081434628.1 flavin reductase family protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 31831.90 Da        Isoelectric Point: 5.5011

>NTDB_id=32783 CTN_RS00445 WP_012644981.1 87267..88151(+) (pilA) [Thermotoga neapolitana DSM 4359]
MGLFDFLKKGLQKTKDAFFGKVVSLLKGKKLDDETREKLEELLIQADVGIETTEYILERLEEKDGNALEALRETLLEILN
FDTALNEPDNPPFVITVVGVNGTGKTTSCGKLAKMFADRGKSVVLAAADTFRAAAIEQLKLWGDRVGATVIAHSEGADPA
AVAFDAVSHALARNKDVVIIDTAGRLHTKKNLMEELRKVHRVIKKKIPEAPHEVLLVIDATTGQNGLVQAKVFKDAVDVT
GIILTKLDGTAKGGIVLAIAKELGIPIKFVGVGEKEDDLKPFDPNAFVEVLLSE

Nucleotide


Download         Length: 885 bp        

>NTDB_id=32783 CTN_RS00445 WP_012644981.1 87267..88151(+) (pilA) [Thermotoga neapolitana DSM 4359]
ATGGGACTCTTTGACTTTCTGAAAAAGGGTCTTCAGAAAACAAAAGATGCGTTCTTTGGAAAAGTGGTGAGTCTTCTTAA
AGGAAAGAAACTCGACGACGAAACCCGCGAAAAACTCGAAGAACTTCTCATTCAGGCAGACGTTGGTATTGAAACAACAG
AGTACATTTTGGAAAGGCTCGAGGAGAAAGATGGAAACGCCCTGGAAGCACTGAGAGAAACCCTTCTTGAAATTCTGAAC
TTCGACACAGCACTGAACGAACCGGACAACCCCCCCTTCGTGATAACCGTGGTCGGTGTGAACGGAACGGGAAAGACCAC
ATCCTGTGGCAAACTCGCAAAGATGTTCGCTGATAGAGGAAAAAGCGTTGTACTGGCAGCCGCCGACACCTTCAGAGCCG
CTGCCATAGAACAGCTCAAACTCTGGGGAGATCGCGTCGGCGCCACCGTGATAGCCCATTCAGAAGGTGCAGATCCCGCT
GCCGTTGCGTTCGATGCCGTCTCCCACGCTCTTGCCAGAAACAAGGACGTGGTGATAATAGACACCGCTGGAAGGCTTCA
CACGAAGAAGAACCTCATGGAAGAACTGAGAAAGGTCCACAGGGTGATAAAGAAAAAGATCCCGGAGGCTCCACACGAGG
TGCTCCTTGTGATAGACGCCACCACAGGGCAGAACGGACTCGTTCAGGCGAAGGTGTTCAAAGATGCGGTGGACGTCACA
GGAATCATTCTGACAAAGCTCGATGGTACCGCAAAGGGTGGAATTGTCCTTGCCATAGCAAAGGAGTTGGGAATTCCCAT
AAAGTTCGTGGGAGTTGGTGAAAAAGAAGACGACCTGAAACCTTTCGACCCCAACGCGTTCGTGGAGGTGCTCCTTTCCG
AATGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(22-64)

SRP54-type protein, GTPase domain (SRP54)

(92-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9KB75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.669

100

0.51

Multiple sequence alignment