Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   EFD77_RS08195 Genome accession   NZ_CP033982
Coordinates   1660544..1661614 (-) Length   356 a.a.
NCBI ID   WP_000776422.1    Uniprot ID   -
Organism   Staphylococcus aureus strain P2D8C1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1655544..1666614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD77_RS08155 (EFD77_08520) gcvPA 1655721..1657067 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EFD77_RS08160 (EFD77_08525) gcvT 1657087..1658178 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EFD77_RS08165 (EFD77_08530) - 1658337..1658861 (-) 525 WP_001015120.1 shikimate kinase -
  EFD77_RS08170 (EFD77_08535) - 1658851..1658997 (-) 147 WP_001792109.1 hypothetical protein -
  EFD77_RS08175 (EFD77_08540) comGF 1659094..1659591 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFD77_RS08180 (EFD77_08545) comGE 1659509..1659808 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  EFD77_RS08185 (EFD77_08550) comGD 1659795..1660241 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFD77_RS08190 (EFD77_08555) comGC 1660219..1660530 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EFD77_RS08195 (EFD77_08560) comGB 1660544..1661614 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFD77_RS08200 (EFD77_08565) comGA 1661586..1662560 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EFD77_RS08205 (EFD77_08570) - 1662612..1663235 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EFD77_RS08210 (EFD77_08575) - 1663232..1663561 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EFD77_RS08215 (EFD77_08580) - 1663561..1664547 (-) 987 WP_000161311.1 ROK family glucokinase -
  EFD77_RS08220 (EFD77_08585) - 1664544..1664747 (-) 204 WP_000087561.1 YqgQ family protein -
  EFD77_RS08225 (EFD77_08590) - 1664728..1666191 (-) 1464 WP_001017852.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41447.84 Da        Isoelectric Point: 10.0606

>NTDB_id=327595 EFD77_RS08195 WP_000776422.1 1660544..1661614(-) (comGB) [Staphylococcus aureus strain P2D8C1]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=327595 EFD77_RS08195 WP_000776422.1 1660544..1661614(-) (comGB) [Staphylococcus aureus strain P2D8C1]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

100

100

1
  comGB Staphylococcus aureus N315

100

100

1

Multiple sequence alignment