Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   EG882_RS05215 Genome accession   NZ_CP033967
Coordinates   988635..989780 (-) Length   381 a.a.
NCBI ID   WP_015240777.1    Uniprot ID   -
Organism   Bacillus velezensis strain 1B-23     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 983635..994780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG882_RS05165 (EG882_05165) speE 983933..984763 (+) 831 WP_124692681.1 spermidine synthase -
  EG882_RS05170 (EG882_05170) speB 984822..985694 (+) 873 WP_095352572.1 agmatinase -
  EG882_RS05175 (EG882_05175) - 985862..986080 (+) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  EG882_RS05185 (EG882_05185) - 986671..987183 (-) 513 WP_031304384.1 stage II sporulation protein M -
  EG882_RS05190 (EG882_05190) - 987227..987508 (-) 282 WP_007407643.1 hypothetical protein -
  EG882_RS05195 (EG882_05195) - 987694..987879 (-) 186 WP_007407642.1 DUF4177 domain-containing protein -
  EG882_RS05200 (EG882_05200) - 987914..988117 (-) 204 WP_007407641.1 hypothetical protein -
  EG882_RS05205 (EG882_05205) - 988131..988334 (-) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  EG882_RS05210 (EG882_05210) phrF 988532..988651 (-) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  EG882_RS05215 (EG882_05215) rapF 988635..989780 (-) 1146 WP_015240777.1 tetratricopeptide repeat protein Regulator
  EG882_RS05225 (EG882_05225) - 990011..991366 (+) 1356 WP_124692682.1 YncE family protein -
  EG882_RS05230 (EG882_05230) - 991406..992782 (+) 1377 WP_015240775.1 YncE family protein -
  EG882_RS05235 (EG882_05235) - 992789..993301 (-) 513 Protein_977 bacteriocin biosynthesis protein AlbG -
  EG882_RS05240 (EG882_05240) - 993455..993883 (+) 429 WP_007407634.1 DUF1934 domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45572.57 Da        Isoelectric Point: 4.5155

>NTDB_id=327448 EG882_RS05215 WP_015240777.1 988635..989780(-) (rapF) [Bacillus velezensis strain 1B-23]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYEIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=327448 EG882_RS05215 WP_015240777.1 988635..989780(-) (rapF) [Bacillus velezensis strain 1B-23]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTTGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACATATTTTTCT
ATGGATTATGCCCGGCAGGCTTATGAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTGGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(190-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment