Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TGR7_RS03895 Genome accession   NC_011901
Coordinates   846723..848438 (-) Length   571 a.a.
NCBI ID   WP_041440910.1    Uniprot ID   -
Organism   Thioalkalivibrio sulfidiphilus HL-EbGr7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 841723..853438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TGR7_RS03870 (Tgr7_0782) - 842315..842788 (+) 474 WP_012637362.1 hypothetical protein -
  TGR7_RS03875 (Tgr7_0783) zapD 842917..843759 (-) 843 WP_049764610.1 cell division protein ZapD -
  TGR7_RS03880 (Tgr7_0784) coaE 843796..844401 (-) 606 WP_012637364.1 dephospho-CoA kinase -
  TGR7_RS03885 (Tgr7_0785) pilD 844532..845398 (-) 867 WP_012637365.1 A24 family peptidase Machinery gene
  TGR7_RS03890 (Tgr7_0786) pilC 845507..846718 (-) 1212 WP_012637366.1 type II secretion system F family protein Machinery gene
  TGR7_RS03895 (Tgr7_0787) pilB 846723..848438 (-) 1716 WP_041440910.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TGR7_RS03900 (Tgr7_0788) - 849041..850843 (+) 1803 WP_012637368.1 ABC transporter ATP-binding protein -
  TGR7_RS16985 (Tgr7_0789) - 850903..851688 (-) 786 WP_187148415.1 polysaccharide pyruvyl transferase family protein -
  TGR7_RS17360 (Tgr7_0790) - 851958..852099 (+) 142 Protein_791 ISL3 family transposase -
  TGR7_RS16990 (Tgr7_0791) - 852144..852941 (-) 798 WP_012637370.1 glycosyltransferase family 25 protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62913.38 Da        Isoelectric Point: 5.3682

>NTDB_id=32737 TGR7_RS03895 WP_041440910.1 846723..848438(-) (pilB) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MSTPAASIPLSGLARRLVQDGLMAEADAGKAVEAAQKKRVPLVAYLVDNKLVESRRVAQAAAEEFGTPVFDLDSFDKELL
PKKLVDEKLVRQHHALPLFKRGNRLFVGIADPMNLTALDEIKFNTGIATEPVLVEQDKLGKLIEESLNDTGAVMEGLMDD
DLDNLEISADDDDRKGEVTESEVDDTPVVRFVNKVLLDAINRKASDIHFEPYEKEFRVRLRMDGVLHEVAKPPVSLSPRI
IARIKVMSRMDISERRIPQDGRIKMQLSKSRAIDFRVNTCPTLYGEKVVLRILDPTSASLGIDVLGYEEEQKKLYLDALS
KPYGMILVTGPTGSGKTVSLYTGLGILNTPDRNISTAEDPAEINMPGINQVNVNPKVGLTFASALRAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPPYNIASSILLIIAQRLARRLCNHCKTVDDVPKDVLLQE
GFTEDDFKDGIKIYKAVGCDQCTNGYKGRVGIYQVLPISEATQRLILEGGNSMQLAEQARKEGINDLRRAGLLKVKAGMT
SLEEINRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=32737 TGR7_RS03895 WP_041440910.1 846723..848438(-) (pilB) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGTCGACACCCGCAGCTTCCATTCCCTTGAGTGGCCTAGCGCGCCGCCTGGTCCAGGACGGGCTAATGGCTGAGGCCGA
CGCCGGAAAGGCGGTTGAGGCTGCACAAAAGAAGCGCGTCCCCCTGGTTGCCTATCTGGTAGACAACAAGCTGGTGGAAT
CCAGGCGGGTCGCTCAGGCCGCCGCCGAGGAGTTCGGCACCCCGGTCTTCGACCTGGATAGCTTCGACAAGGAACTCCTG
CCCAAGAAGCTGGTGGACGAGAAGCTGGTCCGCCAGCACCATGCCCTCCCCCTGTTCAAGCGCGGCAACCGGCTGTTCGT
CGGGATCGCCGACCCCATGAACCTCACCGCCCTGGACGAGATCAAGTTCAACACCGGTATCGCCACCGAACCGGTGCTGG
TGGAACAGGACAAACTGGGCAAGCTGATCGAGGAAAGCCTCAACGACACCGGCGCAGTCATGGAAGGCCTCATGGACGAC
GATCTCGACAACCTCGAGATCAGCGCCGATGACGACGACAGGAAAGGCGAGGTGACGGAAAGCGAGGTGGACGACACCCC
GGTAGTCCGCTTCGTCAACAAGGTGTTACTGGATGCCATCAACCGCAAGGCCTCGGACATTCACTTCGAGCCCTACGAGA
AAGAATTCCGGGTTCGTCTGCGCATGGACGGCGTGCTCCACGAAGTGGCCAAACCCCCAGTATCCCTGTCGCCACGCATC
ATCGCCCGCATCAAGGTCATGTCCCGCATGGACATCTCCGAGCGCCGCATCCCCCAGGACGGGCGCATCAAGATGCAGCT
CTCCAAGAGTCGGGCCATCGACTTTCGTGTCAACACCTGCCCCACGCTCTACGGCGAAAAGGTGGTATTGCGCATCCTCG
ACCCCACCAGCGCCTCCTTAGGCATCGACGTGCTGGGTTATGAGGAAGAACAGAAAAAGCTTTACCTGGATGCCCTGAGC
AAACCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCTCCGGCAAGACCGTCTCCCTTTACACGGGCCTTGGCATCCT
GAACACCCCGGATCGCAACATCTCCACCGCCGAGGACCCGGCCGAGATCAACATGCCCGGGATCAACCAGGTGAACGTCA
ACCCCAAGGTGGGCCTGACCTTCGCCTCTGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAG
ATCCGTGACCTGGAAACCGCCGAGATCGCCATCAAGGCGGCACAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAA
CGACGCCCCCCAGACCCTGACCCGTCTGGCTAATATGGGGGTGCCGCCCTACAACATCGCCTCCTCAATCCTCCTTATCA
TCGCCCAGCGACTTGCCCGGCGGCTGTGCAACCACTGCAAGACCGTCGATGATGTGCCCAAGGATGTGCTGCTACAGGAA
GGGTTCACAGAGGATGACTTTAAGGACGGCATCAAGATCTACAAGGCCGTGGGCTGCGACCAGTGTACCAATGGCTACAA
GGGGCGCGTGGGCATCTACCAGGTGCTGCCCATCTCCGAGGCCACCCAGCGCCTCATCCTGGAGGGTGGCAACTCCATGC
AGCTCGCCGAGCAGGCCCGCAAGGAAGGCATCAACGACCTCCGCCGCGCCGGTCTGCTCAAGGTCAAGGCCGGGATGACC
AGCCTTGAAGAGATCAACCGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.89

99.124

0.564
  pilB Acinetobacter baumannii D1279779

55.497

100

0.557
  pilB Acinetobacter baylyi ADP1

54.783

100

0.552
  pilF Neisseria gonorrhoeae MS11

49.912

99.299

0.496
  pilB Vibrio cholerae strain A1552

51.796

92.644

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

48.313

98.599

0.476
  pilB Vibrio campbellii strain DS40M4

47.35

99.124

0.469
  pilF Thermus thermophilus HB27

38.53

97.723

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

38.727

96.322

0.373
  pilB Haemophilus influenzae Rd KW20

39.808

91.068

0.363

Multiple sequence alignment