Detailed information NTG0326687

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EGM95_RS21390
• Genome accession: NZ_CP033869
• Coordinates: 3847140..3848000 (+)
• Length: 286 a.a.
• NCBI ID: WP_001152284.1
• Uniprot ID: A0A3R9CN06
• Organism: Acinetobacter baumannii strain MRSN15313
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3842140..3853000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGM95_RS18890 (EGM95_18890)	secG	3842745..3843074 (-)	330	WP_000555570.1	preprotein translocase subunit SecG	-
EGM95_RS18895 (EGM95_18895)	tpiA	3843087..3843881 (-)	795	WP_000016939.1	triose-phosphate isomerase	-
EGM95_RS18900 (EGM95_18900)	pilB	3844172..3845884 (+)	1713	WP_057691146.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EGM95_RS18905 (EGM95_18905)	pilC	3845914..3847140 (+)	1227	WP_000279218.1	type II secretion system F family protein	Machinery gene
EGM95_RS21390 (EGM95_18910)	pilD	3847140..3848000 (+)	861	WP_001152284.1	prepilin peptidase	Machinery gene
EGM95_RS18915 (EGM95_18915)	coaE	3848002..3848598 (+)	597	WP_000874127.1	dephospho-CoA kinase	-
EGM95_RS18920 (EGM95_18920)	-	3848595..3849509 (-)	915	WP_000075011.1	DMT family transporter	-
EGM95_RS18925 (EGM95_18925)	rlmB	3849545..3850294 (-)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
EGM95_RS18930 (EGM95_18930)	-	3850396..3850722 (-)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
EGM95_RS18935 (EGM95_18935)	-	3850799..3852070 (-)	1272	WP_052421130.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32189.64 Da
• Isoelectric Point: 7.5058

>NTDB_id=326687 EGM95_RS21390 WP_001152284.1 3847140..3848000(+) (pilD) [Acinetobacter baumannii strain MRSN15313]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPRMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQR
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVIMMFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=326687 EGM95_RS21390 WP_001152284.1 3847140..3848000(+) (pilD) [Acinetobacter baumannii strain MRSN15313]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTAATTTACCGCACGCCAAGAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCCGAGCAACCTATTATTGATCATGAGAAGCTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACGG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCAATCAG
TATCCGCTATCCAGCCATCGAACTACTCACCATGCTATGTTCATTAGTAGTCATCATGATGTTTGGCCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCCTACCTTTAGCTGCGCTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGAGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

• Type IV leader peptidase family (Peptidase_A24) (138-247)

3D structure

• AlphaFold DB: A0A3R9CN06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	98.252	100	0.983
pilD	Acinetobacter baumannii D1279779	97.902	100	0.979
pilD	Vibrio cholerae strain A1552	53.309	95.105	0.507
pilD	Vibrio campbellii strain DS40M4	52.555	95.804	0.503
pilD	Neisseria gonorrhoeae MS11	47.348	92.308	0.437