Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FNE80_RS19975 Genome accession   NZ_CP041437
Coordinates   4090784..4091224 (+) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain STEC005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4085784..4096224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNE80_RS19955 (FNE80_20260) aroP 4086782..4088155 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FNE80_RS19960 (FNE80_20265) ampE 4088198..4089052 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FNE80_RS19965 (FNE80_20270) ampD 4089049..4089600 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FNE80_RS19970 (FNE80_20275) nadC 4089688..4090581 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FNE80_RS19975 (FNE80_20280) pilA 4090784..4091224 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  FNE80_RS19980 (FNE80_20285) pilB 4091234..4092619 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  FNE80_RS19985 (FNE80_20290) hofC 4092609..4093811 (+) 1203 WP_000157234.1 protein transport protein HofC -
  FNE80_RS19990 (FNE80_20295) guaC 4093846..4094889 (-) 1044 WP_001217338.1 GMP reductase -
  FNE80_RS24605 - 4095045..4095089 (-) 45 WP_120795372.1 protein YacM -
  FNE80_RS19995 (FNE80_20300) coaE 4095114..4095734 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=326620 FNE80_RS19975 WP_000360894.1 4090784..4091224(+) (pilA) [Escherichia coli strain STEC005]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=326620 FNE80_RS19975 WP_000360894.1 4090784..4091224(+) (pilA) [Escherichia coli strain STEC005]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377