Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FNE83_RS20930 Genome accession   NZ_CP041431
Coordinates   4217844..4218284 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain STEC316     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4212844..4223284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNE83_RS20910 (FNE83_21315) aroP 4213842..4215215 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FNE83_RS20915 (FNE83_21320) ampE 4215258..4216112 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FNE83_RS20920 (FNE83_21325) ampD 4216109..4216660 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FNE83_RS20925 (FNE83_21330) nadC 4216748..4217641 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FNE83_RS20930 (FNE83_21335) pilA 4217844..4218284 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  FNE83_RS20935 (FNE83_21340) pilB 4218294..4219679 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  FNE83_RS20940 (FNE83_21345) hofC 4219669..4220871 (+) 1203 WP_000157229.1 protein transport protein HofC -
  FNE83_RS20945 (FNE83_21350) guaC 4220906..4221949 (-) 1044 WP_001217338.1 GMP reductase -
  FNE83_RS26540 - 4222105..4222149 (-) 45 WP_120795372.1 protein YacM -
  FNE83_RS20950 (FNE83_21355) coaE 4222174..4222794 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=326379 FNE83_RS20930 WP_000360902.1 4217844..4218284(+) (pilA) [Escherichia coli strain STEC316]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=326379 FNE83_RS20930 WP_000360902.1 4217844..4218284(+) (pilA) [Escherichia coli strain STEC316]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377