Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EGY28_RS20515 Genome accession   NZ_CP033840
Coordinates   1500248..1501753 (-) Length   501 a.a.
NCBI ID   WP_035971708.1    Uniprot ID   A0A892I7Y7
Organism   Burkholderia dolosa strain FDAARGOS_562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1495248..1506753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY28_RS20490 (EGY28_20660) - 1496290..1496925 (-) 636 WP_006763368.1 LON peptidase substrate-binding domain-containing protein -
  EGY28_RS20495 (EGY28_20665) rapZ 1496952..1497860 (-) 909 WP_006763369.1 RNase adapter RapZ -
  EGY28_RS20500 (EGY28_20670) hprK 1497927..1498895 (-) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  EGY28_RS20505 (EGY28_20675) - 1499022..1499534 (-) 513 WP_035971706.1 PTS sugar transporter subunit IIA -
  EGY28_RS20510 (EGY28_20680) hpf 1499730..1500089 (-) 360 WP_006763372.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EGY28_RS20515 (EGY28_20685) rpoN/rpoN1 1500248..1501753 (-) 1506 WP_035971708.1 RNA polymerase factor sigma-54 Machinery gene
  EGY28_RS20520 (EGY28_20690) lptB 1501908..1502684 (-) 777 WP_006763374.1 LPS export ABC transporter ATP-binding protein -
  EGY28_RS20525 (EGY28_20695) lptA 1502681..1503346 (-) 666 WP_006763375.1 lipopolysaccharide transport periplasmic protein LptA -
  EGY28_RS20530 (EGY28_20700) lptC 1503367..1503981 (-) 615 WP_006763376.1 LPS export ABC transporter periplasmic protein LptC -
  EGY28_RS20535 (EGY28_20705) - 1503985..1504521 (-) 537 WP_006763377.1 HAD family hydrolase -
  EGY28_RS20540 (EGY28_20710) kdsD 1504521..1505504 (-) 984 WP_035973266.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54790.95 Da        Isoelectric Point: 4.8740

>NTDB_id=326336 EGY28_RS20515 WP_035971708.1 1500248..1501753(-) (rpoN/rpoN1) [Burkholderia dolosa strain FDAARGOS_562]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWISSPLRVAADGSLIAQTPLAPNAEPQA
TNGSSASSERAERDEPAGADEYDGYGTGDGNDGPQWNLDEFGRANGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALDIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=326336 EGY28_RS20515 WP_035971708.1 1500248..1501753(-) (rpoN/rpoN1) [Burkholderia dolosa strain FDAARGOS_562]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCTCCAGCCCACTGCGCGTCGCGGCGGACGGGTCGCTGATCGCACAGACGCCGCTCGCGCCGAACGCCGAACCTCAAGCG
ACGAACGGCAGCAGCGCGTCGAGCGAGCGCGCCGAACGCGACGAGCCCGCCGGCGCCGACGAATACGACGGCTACGGAAC
CGGCGACGGCAACGACGGCCCGCAATGGAACCTCGACGAATTCGGCCGCGCGAACGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTGCAGGAGGCCGCGACGTCGCTGCGCGATCACCTCGCCGCGCAGCTGCGCGTCACGCAGGCCGGCCCG
CGCGACCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGATGACGACGGCTATCTGACCGCGTCGCTCGACGAAGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCGGCGCTTGCACTGCTGCACAGCTTCGACCCGG
CGGGCGTCGGCGCCCGTTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGTCTCGATCCGTCCCCGACACGCACGCTC
GCGCTGGACATCGTGTCCCAGCACCTGGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCATCTGAAGGCGAC
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCATTCCCCGGCGCCGCATACGGGAAGGCCGAGG
CCGACTACGTCGTCCCGGACATCATCGTGAAAAAAGCCGGCCAGGGATGGCTCGCGGAACTCAACCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGACCGGCTCGCTGAAGCAGCA
GCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGTTTCGACACGATTCTGCGTGTCGCCCAGGCCATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment