Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   IHMA87_RS25680 Genome accession   NZ_CP041354
Coordinates   5330308..5330733 (+) Length   141 a.a.
NCBI ID   WP_034081452.1    Uniprot ID   E9KTN5
Organism   Pseudomonas aeruginosa strain AZPAE15042     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5325308..5335733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHMA87_RS25665 (IHMA87_05014) pilX 5325896..5326483 (+) 588 WP_034081449.1 type 4a pilus minor pilin PilX -
  IHMA87_RS25670 (IHMA87_05015) pilY1 5326495..5329962 (+) 3468 WP_034081450.1 type 4a pilus biogenesis protein PilY1 -
  IHMA87_RS25675 (IHMA87_05016) pilY2 5329964..5330311 (+) 348 WP_012077312.1 type 4a fimbrial biogenesis protein PilY2 -
  IHMA87_RS25680 (IHMA87_05017) comF 5330308..5330733 (+) 426 WP_034081452.1 type IV pilin protein Machinery gene
  IHMA87_RS25685 (IHMA87_05018) ispH 5330782..5331726 (-) 945 WP_034081453.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  IHMA87_RS25690 (IHMA87_05019) fkpB 5331812..5332252 (-) 441 WP_003150012.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  IHMA87_RS25695 (IHMA87_05020) lspA 5332245..5332754 (-) 510 WP_034081454.1 signal peptidase II -
  IHMA87_RS25700 (IHMA87_05021) ileS 5332747..5335578 (-) 2832 WP_034081455.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15256.32 Da        Isoelectric Point: 9.3371

>NTDB_id=325635 IHMA87_RS25680 WP_034081452.1 5330308..5330733(+) (comF) [Pseudomonas aeruginosa strain AZPAE15042]
MTKKEKGFTLLEMMIVVVLIGILLGIAIPSYQNYVIRANRTEGQALLSDAAARQERYYSQNPGVGYTNDVAKLGMSSANS
PNNLYSLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNDSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=325635 IHMA87_RS25680 WP_034081452.1 5330308..5330733(+) (comF) [Pseudomonas aeruginosa strain AZPAE15042]
ATGACGAAAAAAGAGAAAGGCTTCACCTTGCTGGAGATGATGATCGTCGTGGTGCTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCGCCAACCGCACCGAAGGCCAGGCGCTGCTCTCGGACGCGGCGGCACGCC
AGGAGCGCTACTATTCGCAGAACCCCGGGGTCGGTTACACCAACGACGTGGCCAAGCTGGGCATGAGCTCGGCCAACTCG
CCGAACAATCTGTACAGCCTCACCATAGCGACGCCCACCAGTACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACTCTCAACCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGTAACGACA
GCACGGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E9KTN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.52

90.071

0.383
  pilE Legionella pneumophila str. Paris

38.571

99.291

0.383
  pilE Legionella pneumophila strain ERS1305867

38.571

99.291

0.383