Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EEX22_RS06200 Genome accession   NZ_CP033598
Coordinates   1362371..1362916 (-) Length   181 a.a.
NCBI ID   WP_156732369.1    Uniprot ID   -
Organism   Pasteurella multocida strain CQ7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357371..1367916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEX22_RS06175 (EEX22_06210) - 1357581..1358018 (-) 438 WP_010907449.1 methyltransferase family protein -
  EEX22_RS06180 (EEX22_06215) - 1358231..1358842 (-) 612 WP_005725137.1 YfgM family protein -
  EEX22_RS06185 (EEX22_06220) hisS 1358861..1360132 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  EEX22_RS06190 (EEX22_06225) ispG 1360162..1361265 (-) 1104 WP_170345828.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EEX22_RS06195 (EEX22_06230) - 1361292..1362248 (-) 957 WP_156732368.1 helix-turn-helix domain-containing protein -
  EEX22_RS06200 (EEX22_06235) pilF/pilF2 1362371..1362916 (-) 546 WP_156732369.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EEX22_RS06205 (EEX22_06240) - 1362956..1364140 (-) 1185 WP_156732370.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EEX22_RS06210 (EEX22_06245) - 1364320..1366578 (-) 2259 WP_170345837.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20951.58 Da        Isoelectric Point: 8.2542

>NTDB_id=324187 EEX22_RS06200 WP_156732369.1 1362371..1362916(-) (pilF/pilF2) [Pasteurella multocida strain CQ7]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALKAPYYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=324187 EEX22_RS06200 WP_156732369.1 1362371..1362916(-) (pilF/pilF2) [Pasteurella multocida strain CQ7]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAAGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAAAGCACCTT
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50

97.238

0.486
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459

Multiple sequence alignment