Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   D5285_RS13710 Genome accession   NZ_CP033389
Coordinates   2714595..2715659 (-) Length   354 a.a.
NCBI ID   WP_020452178.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CBMAI 1303     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2709595..2720659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5285_RS13665 (D5285_13665) tapA 2710089..2710817 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  D5285_RS13670 (D5285_13670) - 2711095..2711415 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  D5285_RS13675 (D5285_13675) - 2711445..2711627 (-) 183 WP_020452171.1 YqzE family protein -
  D5285_RS13680 (D5285_13680) comGG 2711716..2712081 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  D5285_RS13685 (D5285_13685) comGF 2712093..2712581 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  D5285_RS13690 (D5285_13690) comGE 2712490..2712837 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  D5285_RS13695 (D5285_13695) comGD 2712821..2713258 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  D5285_RS13700 (D5285_13700) comGC 2713264..2713557 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  D5285_RS13705 (D5285_13705) comGB 2713571..2714605 (-) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  D5285_RS13710 (D5285_13710) comGA 2714595..2715659 (-) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  D5285_RS13715 (D5285_13715) - 2715817..2716656 (-) 840 WP_020452179.1 STAS domain-containing protein -
  D5285_RS13725 (D5285_13725) - 2716898..2717275 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D5285_RS13730 (D5285_13730) - 2717486..2717731 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  D5285_RS13735 (D5285_13735) - 2717767..2718405 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  D5285_RS13740 (D5285_13740) - 2718561..2718734 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  D5285_RS13745 (D5285_13745) - 2718795..2719109 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -
  D5285_RS13750 (D5285_13750) - 2719125..2720234 (-) 1110 WP_020452183.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39806.27 Da        Isoelectric Point: 9.1063

>NTDB_id=323572 D5285_RS13710 WP_020452178.1 2714595..2715659(-) (comGA) [Bacillus paralicheniformis strain CBMAI 1303]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKVSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDNCSLYCRLSRPVRRASIFELLYGKSLNLCIEEAKGRC
GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=323572 D5285_RS13710 WP_020452178.1 2714595..2715659(-) (comGA) [Bacillus paralicheniformis strain CBMAI 1303]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTGACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGGTGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATCGAATAC
GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTCG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC
AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAACTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTGAGAAGAGCAAGCATCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
GGTGACATAAAAACAGAAACATTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.514

100

0.675
  pilB Vibrio cholerae strain A1552

38.671

93.503

0.362

Multiple sequence alignment