Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   D5285_RS13700 Genome accession   NZ_CP033389
Coordinates   2713264..2713557 (-) Length   97 a.a.
NCBI ID   WP_020452176.1    Uniprot ID   A0A6N2FHJ1
Organism   Bacillus paralicheniformis strain CBMAI 1303     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2708264..2718557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5285_RS13655 (D5285_13655) tasA 2708641..2709435 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  D5285_RS13660 (D5285_13660) sipW 2709508..2710092 (-) 585 WP_020452168.1 signal peptidase I SipW -
  D5285_RS13665 (D5285_13665) tapA 2710089..2710817 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  D5285_RS13670 (D5285_13670) - 2711095..2711415 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  D5285_RS13675 (D5285_13675) - 2711445..2711627 (-) 183 WP_020452171.1 YqzE family protein -
  D5285_RS13680 (D5285_13680) comGG 2711716..2712081 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  D5285_RS13685 (D5285_13685) comGF 2712093..2712581 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  D5285_RS13690 (D5285_13690) comGE 2712490..2712837 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  D5285_RS13695 (D5285_13695) comGD 2712821..2713258 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  D5285_RS13700 (D5285_13700) comGC 2713264..2713557 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  D5285_RS13705 (D5285_13705) comGB 2713571..2714605 (-) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  D5285_RS13710 (D5285_13710) comGA 2714595..2715659 (-) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  D5285_RS13715 (D5285_13715) - 2715817..2716656 (-) 840 WP_020452179.1 STAS domain-containing protein -
  D5285_RS13725 (D5285_13725) - 2716898..2717275 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D5285_RS13730 (D5285_13730) - 2717486..2717731 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  D5285_RS13735 (D5285_13735) - 2717767..2718405 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10670.54 Da        Isoelectric Point: 9.1200

>NTDB_id=323570 D5285_RS13700 WP_020452176.1 2713264..2713557(-) (comGC) [Bacillus paralicheniformis strain CBMAI 1303]
MNEKGFTLIEMLVVMLVISILLLITIPNVTKHNQSIQKKGCEGLINMVQAQITAYQMDHDGKTPSRADLESEGYVKKNLK
CPNGKAIKISNGKAQAD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=323570 D5285_RS13700 WP_020452176.1 2713264..2713557(-) (comGC) [Bacillus paralicheniformis strain CBMAI 1303]
GTGAATGAAAAGGGATTTACACTGATAGAAATGTTAGTCGTCATGCTCGTCATCTCCATCCTGCTGTTGATCACCATACC
GAATGTGACCAAGCATAATCAAAGTATTCAAAAGAAAGGATGCGAAGGGTTAATTAATATGGTTCAAGCGCAAATCACGG
CTTATCAAATGGATCATGACGGGAAAACGCCGAGTAGGGCGGACCTTGAATCTGAAGGTTACGTGAAGAAGAACTTAAAG
TGCCCGAATGGGAAAGCAATTAAAATTTCAAACGGCAAAGCACAGGCTGATTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N2FHJ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

71.134

100

0.711
  comGC Staphylococcus aureus MW2

45.263

97.938

0.443
  comGC Staphylococcus aureus N315

45.263

97.938

0.443

Multiple sequence alignment