Detailed information    

experimental Experimentally validated

Overview

Name   HI0659   Type   Machinery gene
Locus tag   HI_0659 Genome accession   L42023
Coordinates   702730..703026 (-) Length   98 a.a.
NCBI ID   AAC22323.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   DNA uptake   
DNA binding and uptake

Function

The deletion of HI0659 eliminated DNA uptake and transformation.


Genomic Context

Location: 697730..708026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0654 - 698164..698721 (-) 558 AAC22313.1 DNA-3-methyladenine glycosidase I (tagI) -
  HI_0655 - 698718..699536 (-) 819 AAC22314.1 shikimate 5-dehydrogenase (aroE) -
  HI_0656 - 699540..700091 (-) 552 AAC22315.1 conserved hypothetical protein -
  HI_0656.1 - 700107..700643 (-) 537 AAC22316.1 conserved hypothetical protein -
  HI_0658 - 700653..702569 (-) 1917 AAC22317.1 ABC transporter, ATP-binding protein -
  HI_0659 HI0659 702730..703026 (-) 297 AAC22323.1 predicted coding region HI0659 Machinery gene
  HI_0660 - 703019..703378 (-) 360 AAC22324.1 predicted coding region HI0660 -
  HI_0661 - 703648..706680 (-) 3033 AAC22319.1 hemoglobin-binding protein -

Sequence

Protein


Download         Length: 98 a.a.        Molecular weight: 11092.94 Da        Isoelectric Point: 10.3139

>NTDB_id=1360 HI_0659 AAC22323.1 702730..703026(-) (HI0659) [Haemophilus influenzae Rd KW20]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide


Download         Length: 297 bp        

>NTDB_id=1360 HI_0659 AAC22323.1 702730..703026(-) (HI0659) [Haemophilus influenzae Rd KW20]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTTCCTCTAAGGGTAAAAAATGCCTAA

Domains

Predicted by InterproScan.

Helix-turn-helix (HTH_3)

(41-89)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]