Detailed information NTG0032348

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PLES_RS02020
• Genome accession: NC_011770
• Coordinates: 431523..432671 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa LESB58
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 426523..437671

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PLES_RS01995	-	427674..427970 (-)	297	WP_024082404.1	DUF167 domain-containing protein	-
PLES_RS02000 (PLES_03901)	-	427967..428560 (-)	594	WP_003084544.1	YggT family protein	-
PLES_RS02005 (PLES_03911)	proC	428571..429392 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PLES_RS02010 (PLES_03921)	-	429404..430096 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PLES_RS02015 (PLES_03931)	pilT	430311..431345 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PLES_RS02020 (PLES_03941)	pilU	431523..432671 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PLES_RS02025 (PLES_03951)	czcD	432679..433578 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PLES_RS02030 (PLES_03961)	-	433732..434136 (+)	405	WP_003084560.1	NINE protein	-
PLES_RS02035 (PLES_03971)	-	434379..435752 (+)	1374	WP_012613501.1	pyridoxal-phosphate dependent enzyme	-
PLES_RS02040 (PLES_03981)	-	435749..436933 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=32348 PLES_RS02020 WP_003100959.1 431523..432671(+) (pilU) [Pseudomonas aeruginosa LESB58]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=32348 PLES_RS02020 WP_003100959.1 431523..432671(+) (pilU) [Pseudomonas aeruginosa LESB58]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382