Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FG899_RS03940 Genome accession   NZ_CP040799
Coordinates   908911..910611 (+) Length   566 a.a.
NCBI ID   WP_012382531.1    Uniprot ID   B2IA49
Organism   Xylella fastidiosa subsp. fastidiosa strain Bakersfield-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 903911..915611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FG899_RS03930 (FG899_04110) - 904027..904938 (+) 912 WP_004088966.1 AEC family transporter -
  FG899_RS03935 (FG899_04115) - 905624..908617 (+) 2994 WP_011097760.1 YadA-like family protein -
  FG899_RS03940 (FG899_04120) pilF 908911..910611 (+) 1701 WP_012382531.1 type II secretion system ATPase GspE Machinery gene
  FG899_RS03945 (FG899_04125) - 910647..911864 (+) 1218 WP_004088957.1 type II secretion system F family protein -
  FG899_RS03950 (FG899_12190) - 911898..912071 (+) 174 WP_014607693.1 hypothetical protein -
  FG899_RS03955 (FG899_04130) gspG 912082..912516 (+) 435 WP_004088955.1 type II secretion system major pseudopilin GspG -
  FG899_RS03960 (FG899_04135) - 912522..913028 (+) 507 WP_004083685.1 Tfp pilus assembly protein FimT/FimU -
  FG899_RS03965 (FG899_04140) - 913025..913423 (+) 399 WP_004088953.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FG899_RS03970 (FG899_04145) - 913420..914046 (+) 627 WP_011097763.1 type II secretion system protein J -
  FG899_RS03975 (FG899_04150) - 914046..914891 (+) 846 WP_011097764.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62713.53 Da        Isoelectric Point: 5.0845

>NTDB_id=323385 FG899_RS03940 WP_012382531.1 908911..910611(+) (pilF) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-1]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPEFSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAVLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPTDDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFTDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPMHAERYLASPEQIARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDALRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=323385 FG899_RS03940 WP_012382531.1 908911..910611(+) (pilF) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-1]
ATGAATGTAAGTATGGAAGCATCCATCGTTGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGTGATCTGTATCGTGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGTTGGGCCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTCTCGGCG
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACAGGTGCAGTATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAAGGCCGCAGCGTCATGGGCATGATCGTGGAGGCG
ACCGATGGTGATGCGGCGCCCACAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATTCAGCGTGCGGTTGAATTACGCGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATTATGGCCAAACTTAATATTGCTGAACGCCGCTTGCCCCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGACCGGGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTACCGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTA
CTGGTCACGGGTCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATTGAAGGCATCAATCAGATCCAGGCGAAGCCGCAGATCGGGCTGG
ATTTTGCCAGCGCACTACGCAGCATTGTGCGTCAGGATCCGGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCGGCGCTGACAGGTCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTGGATATGGGTGTGGAGGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTATGCATGCTGAGCGTTATCTTGCTTCGCCAGAGCAGATCGCGCGTTTTGATTTGCGCCGTTTACAG
CCAGAGGGTGATATTTATTTGTACCGTCCGCGCGCTTCAGCGCTTGCGCCCACTGGGTATCTGGGCCGTACGGCGATCGT
CGAATTTCTGATTATGGATGATGCGCTGCGCCGCGCGGTGATCAGCCGTACCGGCATAGGGGAGTTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACCACGATTGAAGAGGTCTTG
CGTGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)

Type II/IV secretion system protein (T2SSE)

(189-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2IA49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.761

97.527

0.398
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

39.518

95.23

0.376
  pilB Legionella pneumophila strain ERS1305867

36.731

100

0.369

Multiple sequence alignment