Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EB816_RS07355 Genome accession   NZ_CP033337
Coordinates   1419904..1421229 (-) Length   441 a.a.
NCBI ID   WP_023611937.1    Uniprot ID   A0A5S4TFE6
Organism   Streptococcus pyogenes strain TSPY453     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1414904..1426229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB816_RS07345 (EB816_07345) raiA 1418639..1419187 (-) 549 WP_023611925.1 ribosome-associated translation inhibitor RaiA -
  EB816_RS07350 (EB816_07350) - 1419267..1419932 (-) 666 Protein_1362 ComF family protein -
  EB816_RS07355 (EB816_07355) comFA/cflA 1419904..1421229 (-) 1326 WP_023611937.1 DEAD/DEAH box helicase Machinery gene
  EB816_RS07360 (EB816_07360) - 1421285..1421917 (+) 633 WP_032465539.1 YigZ family protein -
  EB816_RS07365 (EB816_07365) cysK 1422045..1422986 (+) 942 WP_011888673.1 cysteine synthase A -
  EB816_RS07370 (EB816_07370) - 1423004..1423381 (-) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  EB816_RS07375 (EB816_07375) - 1423381..1424781 (-) 1401 WP_023611922.1 Cof-type HAD-IIB family hydrolase -
  EB816_RS07380 (EB816_07380) - 1424818..1425459 (-) 642 WP_002983671.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49818.36 Da        Isoelectric Point: 10.0009

>NTDB_id=323141 EB816_RS07355 WP_023611937.1 1419904..1421229(-) (comFA/cflA) [Streptococcus pyogenes strain TSPY453]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWGGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKTFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=323141 EB816_RS07355 WP_023611937.1 1419904..1421229(-) (comFA/cflA) [Streptococcus pyogenes strain TSPY453]
ATGGAGTCTATTGAAAATAGTTATGGACGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATTGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGGGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGACTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTGACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGAAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(98-244)

Helicase conserved C-terminal domain (Helicase_C)

(289-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TFE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae D39

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae R6

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae TIGR4

53.938

95.011

0.512
  comFA/cflA Streptococcus mitis NCTC 12261

52.607

95.692

0.503
  comFA/cflA Streptococcus mitis SK321

51.659

95.692

0.494
  comFA Lactococcus lactis subsp. cremoris KW2

47.475

89.796

0.426
  comFA Latilactobacillus sakei subsp. sakei 23K

39.08

98.639

0.385

Multiple sequence alignment