Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EB817_RS02935 Genome accession   NZ_CP033336
Coordinates   546157..547482 (+) Length   441 a.a.
NCBI ID   WP_047235723.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY165     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541157..552482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB817_RS02910 (EB817_02915) - 541927..542568 (+) 642 WP_002983671.1 response regulator transcription factor -
  EB817_RS02915 (EB817_02920) - 542605..544005 (+) 1401 WP_129304529.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  EB817_RS02920 (EB817_02925) - 544005..544382 (+) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  EB817_RS02925 (EB817_02930) cysK 544400..545341 (-) 942 WP_009880467.1 cysteine synthase A -
  EB817_RS02930 (EB817_02935) - 545469..546101 (-) 633 WP_009880468.1 YigZ family protein -
  EB817_RS02935 (EB817_02940) comFA/cflA 546157..547482 (+) 1326 WP_047235723.1 DEAD/DEAH box helicase Machinery gene
  EB817_RS02940 (EB817_02945) - 547454..548119 (+) 666 WP_002983685.1 ComF family protein -
  EB817_RS02945 (EB817_02950) raiA 548199..548747 (+) 549 WP_002988974.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49772.32 Da        Isoelectric Point: 9.9935

>NTDB_id=323072 EB817_RS02935 WP_047235723.1 546157..547482(+) (comFA/cflA) [Streptococcus pyogenes strain TSPY165]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEIISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=323072 EB817_RS02935 WP_047235723.1 546157..547482(+) (comFA/cflA) [Streptococcus pyogenes strain TSPY165]
ATGGAGTCTATTGAAAATAGTTATGGACGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATTGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATAATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAAAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(99-244)

Helicase conserved C-terminal domain (Helicase_C)

(289-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae D39

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae R6

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

54.177

95.011

0.515
  comFA/cflA Streptococcus mitis NCTC 12261

52.844

95.692

0.506
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.727

89.796

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.31

98.639

0.388

Multiple sequence alignment