Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VS_RS13515 Genome accession   NC_011753
Coordinates   3023134..3023763 (-) Length   209 a.a.
NCBI ID   WP_012604947.1    Uniprot ID   B7VLK0
Organism   Vibrio atlanticus     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3018134..3028763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VS_RS13495 (VS_2871) aroB 3019067..3020155 (-) 1089 WP_012604944.1 3-dehydroquinate synthase -
  VS_RS13500 (VS_2872) aroK 3020180..3020698 (-) 519 WP_009848907.1 shikimate kinase AroK -
  VS_RS13505 (VS_2873) pilQ 3020875..3022593 (-) 1719 WP_012604945.1 type IV pilus secretin PilQ Machinery gene
  VS_RS13510 (VS_2874) pilP 3022629..3023147 (-) 519 WP_041472933.1 pilus assembly protein PilP Machinery gene
  VS_RS13515 (VS_2875) pilO 3023134..3023763 (-) 630 WP_012604947.1 type 4a pilus biogenesis protein PilO Machinery gene
  VS_RS13520 (VS_2876) pilN 3023756..3024370 (-) 615 WP_041472934.1 PilN domain-containing protein Machinery gene
  VS_RS13525 (VS_2877) pilM 3024354..3025379 (-) 1026 WP_041472935.1 type IV pilus assembly protein PilM Machinery gene
  VS_RS13530 (VS_2878) - 3025531..3028089 (+) 2559 WP_012604950.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24167.67 Da        Isoelectric Point: 4.4907

>NTDB_id=32299 VS_RS13515 WP_012604947.1 3023134..3023763(-) (pilO) [Vibrio atlanticus]
MANLQSRISWQNSVSLQDLDADEITEWPLLPQLLVILLLMILIQGVGTWLYVLPLDDELQQMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNALTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDEIQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=32299 VS_RS13515 WP_012604947.1 3023134..3023763(-) (pilO) [Vibrio atlanticus]
ATGGCTAACCTTCAAAGTAGGATTAGCTGGCAAAATAGTGTGAGCTTGCAAGATCTCGATGCTGATGAGATCACCGAATG
GCCGTTGTTGCCCCAACTGCTCGTTATTCTGTTGCTGATGATATTAATCCAAGGTGTCGGGACTTGGCTGTATGTGCTGC
CTTTAGACGATGAACTGCAGCAGATGAAGCAGCAAGAGCAGACTTTGAAAGCAACATTAAGGATAAAAGCGAACAAGGTT
GCGGCTTTGCCTAAGCTTCAGAGTCAGTTGGATGAGCTAACCAGTCGCTACGACTATCTGTTAGAACAGTTGCCAGTTCA
AAAGGAGCTCGCCAGTATGTTGGCCTCAGTGAATGAACTTGGTTTGGATAATGCGCTAACCTTTACGCGCATTGATTGGG
GGCAAAAGCAGAACAAGGAATTCCTCTATCGGTTACCCCTCAATATTGAGCTAACCGGTGATTACCACGAGATTGGCGAT
TTCTCCGCGGCCATCGCAAAACTGCCGCGCATTATTAGCTTCGATGATGTGAATTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTACATTTCAGGGTTCGAGCTTACACCTATCAGTTTAAATCGGAGGTGAATGATGAAATTCAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7VLK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.804

92.823

0.713
  pilO Vibrio campbellii strain DS40M4

72.34

89.952

0.651

Multiple sequence alignment