Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   D9Y32_RS21060 Genome accession   NZ_CP033218
Coordinates   4030130..4031194 (-) Length   354 a.a.
NCBI ID   WP_003183477.1    Uniprot ID   Q8VQ73
Organism   Bacillus licheniformis strain TCCC 11148     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4025130..4036194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9Y32_RS21015 (D9Y32_21040) tapA 4025631..4026359 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  D9Y32_RS21020 (D9Y32_21045) - 4026636..4026956 (+) 321 WP_003183454.1 YqzG/YhdC family protein -
  D9Y32_RS21025 (D9Y32_21050) - 4026980..4027162 (-) 183 WP_003183456.1 YqzE family protein -
  D9Y32_RS21030 (D9Y32_21055) comGG 4027250..4027615 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  D9Y32_RS21035 (D9Y32_21060) comGF 4027628..4028116 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  D9Y32_RS21040 (D9Y32_21065) comGE 4028025..4028372 (-) 348 WP_009327907.1 competence type IV pilus minor pilin ComGE -
  D9Y32_RS21045 (D9Y32_21070) comGD 4028356..4028793 (-) 438 WP_009327905.1 competence type IV pilus minor pilin ComGD -
  D9Y32_RS21050 (D9Y32_21075) comGC 4028799..4029092 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  D9Y32_RS21055 (D9Y32_21080) comGB 4029106..4030140 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  D9Y32_RS21060 (D9Y32_21085) comGA 4030130..4031194 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  D9Y32_RS21065 (D9Y32_21090) - 4031351..4032190 (-) 840 WP_061576836.1 STAS domain-containing protein -
  D9Y32_RS21075 (D9Y32_21100) - 4032413..4032808 (-) 396 WP_142782738.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D9Y32_RS21080 (D9Y32_21105) - 4033017..4033262 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  D9Y32_RS21085 (D9Y32_21110) - 4033300..4033938 (-) 639 WP_003183485.1 MBL fold metallo-hydrolase -
  D9Y32_RS21090 (D9Y32_21115) - 4034094..4034267 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  D9Y32_RS21095 (D9Y32_21120) - 4034328..4034642 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -
  D9Y32_RS21100 (D9Y32_21125) - 4034658..4035767 (-) 1110 WP_003183488.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39727.31 Da        Isoelectric Point: 9.1029

>NTDB_id=322758 D9Y32_RS21060 WP_003183477.1 4030130..4031194(-) (comGA) [Bacillus licheniformis strain TCCC 11148]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDGLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINREPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIDY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLELGVDMTEIEQTLVAVSAQRLVDIVCPFCGDSCTLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GGIKTDTLKMLIRKGIALGYLPSKTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=322758 D9Y32_RS21060 WP_003183477.1 4030130..4031194(-) (comGA) [Bacillus licheniformis strain TCCC 11148]
TTGTACACGATCGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTACGCTTCAGGATTGATGACGAACTGTTTCAAAAAGACGGATTAACGAGAAATGAGTGCT
CTAGACTCATTTCTCACTTTAAATTTCTTTCTTCGATGGACATTGGCGAGAGACGGCTGCCGCAAAGCGGGGCTTTAACT
CTTTATATCAACCGTGAGCCCGTTCATTTAAGGATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTCCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTTTTCACCGGCCCGACAGGCTCGGGAAAAACGACAACACTCTATTCTTTAATTGACTAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACGTTGGAAGACCCGGTCGAATCAAGAAGCGAAAACATTTTGCAAGTACA
AGTGAACGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTGCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACGGGACACCTTGTTCTATCGAGCATGCAT
GCGAAAAATGCTAAAGGTGCGATATACAGGCTGCTCGAGCTCGGCGTTGATATGACAGAAATTGAACAAACATTGGTCGC
AGTCAGCGCCCAGCGTCTTGTCGATATCGTCTGTCCGTTTTGCGGCGACAGCTGCACTTTGTATTGCAGATTGTCGAGAC
CAGTTAGAAGAGCAAGCGTCTTTGAACTTTTGTACGGAAAAAGCCTTAATCTCTGCATCGAAGAAGCAAAAGGCAGATGC
GGTGGCATCAAAACAGACACCTTAAAAATGCTGATCCGCAAGGGAATAGCACTCGGATATCTGCCGTCGAAAACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

66.949

100

0.669

Multiple sequence alignment